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Potency of an inactivated avian influenza vaccine prepared from a non-pathogenic H5N1 reassortant virus generated between isolates from migratory ducks in Asia.
- Source :
-
Archives of virology [Arch Virol] 2008; Vol. 153 (9), pp. 1685-92. Date of Electronic Publication: 2008 Jul 25. - Publication Year :
- 2008
-
Abstract
- A reassortant influenza virus, A/duck/Hokkaido/Vac-1/2004 (H5N1) (Dk/Vac-1/04), was generated between non-pathogenic avian influenza viruses isolated from migratory ducks in Asia. Dk/Vac-1/04 (H5N1) virus particles propagated in embryonated chicken eggs were inactivated with formalin and adjuvanted with mineral oil to form a water-in-oil emulsion. The resulting vaccine was injected intramuscularly into chickens. The chickens were challenged with either of the highly pathogenic avian influenza virus strains A/chicken/Yamaguchi/7/2004 (H5N1) or A/swan/Mongolia/3/2005 (H5N1) at 21 days post-vaccination (p. v.), when the geometric mean serum HI titers of the birds was 64 with the challenge virus strains. The vaccinated chickens were protected from manifestation of disease signs upon challenge with either of the highly pathogenic avian influenza viruses. However, challenge virus was recovered at low titers from the birds at 2 and 4 days post-challenge (p.c.). All 3 chickens challenged at 6 days p.v. died, whereas 3 chickens challenged at 8 days p.v. survived. These results indicate that the present vaccine confers clinical protection and reduction of virus shedding against highly pathogenic avian influenza virus challenge and should be useful as an optional tool in emergency cases.
- Subjects :
- Animal Migration
Animals
Animals, Wild
Antibodies, Viral blood
Asia
Chick Embryo
Chickens
Influenza A Virus, H5N1 Subtype genetics
Influenza A Virus, H5N1 Subtype isolation & purification
Influenza A virus immunology
Influenza Vaccines administration & dosage
Influenza Vaccines genetics
Influenza in Birds immunology
Influenza in Birds virology
Reassortant Viruses genetics
Reassortant Viruses isolation & purification
Vaccines, Inactivated administration & dosage
Vaccines, Inactivated genetics
Virus Shedding
Ducks virology
Influenza A Virus, H5N1 Subtype immunology
Influenza Vaccines immunology
Influenza in Birds prevention & control
Reassortant Viruses immunology
Vaccines, Inactivated immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0304-8608
- Volume :
- 153
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 18651092
- Full Text :
- https://doi.org/10.1007/s00705-008-0171-1