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Nesfatin-1 inhibits NPY neurons in the arcuate nucleus.
- Source :
-
Brain research [Brain Res] 2008 Sep 16; Vol. 1230, pp. 99-106. Date of Electronic Publication: 2008 Jul 01. - Publication Year :
- 2008
-
Abstract
- Although the novel satiety factor nesfatin-1 has been shown to influence feeding behavior through effects on melanocortin signaling, the specific hypothalamic neuronal substrates through which such effects are mediated have yet to be elucidated. To identify neuronal cell types potentially important in mediating nesfatin-1's effects, whole cell current clamp recordings were made from hypothalamic arcuate nucleus neurons and the effects of bath applied nesfatin-1 on membrane excitability determined. Neurons then underwent phenotypic identification post hoc using single cell RT-PCR. Nesfatin-1 (10 nM) had effects on the majority of arcuate nucleus neurons tested, with most responsive cells hyperpolarizing following exposure. Furthermore, 9 of 11 identified NPY neurons hyperpolarized in response to nesfatin-1 exposure. Pharmacological experiments revealed that glibenclamide (500 nM), an ATP-sensitive potassium conductance antagonist, prevented nesfatin-1-induced hyperpolarization. Therefore, nesfatin-1-induced inhibition of feeding may be mediated through the inhibition of orexigenic NPY neurons.
- Subjects :
- Anesthetics, Local pharmacology
Animals
Arcuate Nucleus of Hypothalamus cytology
Arcuate Nucleus of Hypothalamus drug effects
Calcium-Binding Proteins
DNA-Binding Proteins
Electrophysiology
Glyburide pharmacology
Hypothalamus cytology
Hypothalamus drug effects
In Vitro Techniques
KATP Channels drug effects
Male
Nerve Tissue Proteins antagonists & inhibitors
Nucleobindins
Potassium Channel Blockers pharmacology
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Tetrodotoxin pharmacology
Arcuate Nucleus of Hypothalamus physiology
Nerve Tissue Proteins pharmacology
Neurons physiology
Neuropeptide Y physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-8993
- Volume :
- 1230
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 18625211
- Full Text :
- https://doi.org/10.1016/j.brainres.2008.06.084