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Boosting systemic and secreted antibody responses in mice orally immunized with recombinant Bacillus subtilis strains following parenteral priming with a DNA vaccine encoding the enterotoxigenic Escherichia coli (ETEC) CFA/I fimbriae B subunit.
- Source :
-
Vaccine [Vaccine] 2008 Jul 29; Vol. 26 (32), pp. 3998-4005. Date of Electronic Publication: 2008 Jun 02. - Publication Year :
- 2008
-
Abstract
- Recombinant Bacillus subtilis strains, either spores or vegetative cells, may be employed as safe and low cost orally delivered live vaccine vehicles. In this study, we report the use of an orally delivered B. subtilis vaccine strain to boost systemic and secreted antibody responses in mice i.m. primed with a DNA vaccine encoding the structural subunit (CfaB) of the CFA/I fimbriae encoded by enterotoxigenic Escherichia coli (ETEC), an important etiological agent of diarrhea among travelers and children living in endemic regions. DBA/2 female mice submitted to the prime-boost immunization regimen developed synergic serum (IgG) and mucosal (IgA) antibody responses to the target CfaB antigen. Moreover, in contrast to mice immunized only with one vaccine formulation, sera harvested from prime-boosted vaccinated individuals inhibited adhesion of ETEC cells to human red blood cells. Additionally, vaccinated dams conferred full passive protection to suckling newborn mice challenged with a virulent ETEC strain. Taken together the present results further demonstrate the potential use of recombinant B. subtilis strains as an alternative live vaccine vehicle.
- Subjects :
- Administration, Oral
Animals
Bacillus subtilis genetics
Enterotoxigenic Escherichia coli genetics
Female
Fimbriae Proteins genetics
Gene Expression Regulation, Bacterial
Humans
Immunization
Infusions, Parenteral
Mice
Mice, Inbred DBA
Promoter Regions, Genetic genetics
Recombinant Proteins immunology
Survival Analysis
Vaccines, DNA administration & dosage
Antibodies, Bacterial blood
Bacillus subtilis immunology
Bacterial Vaccines immunology
Drug Delivery Systems
Enterotoxigenic Escherichia coli immunology
Fimbriae Proteins immunology
Vaccines, DNA immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 26
- Issue :
- 32
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 18597902
- Full Text :
- https://doi.org/10.1016/j.vaccine.2008.05.030