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Mechanisms of murine lacrimal gland repair after experimentally induced inflammation.
- Source :
-
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2008 Oct; Vol. 49 (10), pp. 4399-406. Date of Electronic Publication: 2008 Jun 27. - Publication Year :
- 2008
-
Abstract
- Purpose: The authors recently reported that a severe inflammatory response resulting in substantial loss of acinar cells was induced by a single injection of interleukin-1alpha into the lacrimal gland and that this effect was reversible. The purpose of the present study was to determine the mechanisms involved in lacrimal gland injury and repair.<br />Methods: Inflammation was induced by direct injection of recombinant human interleukin-1alpha (IL-1alpha, 1 microg in 2 microL) into the exorbital lacrimal glands of anesthetized female BALB/c mice. Animals were killed 1, 2, 3, 4, 5, 6, or 7 days after injection. Exorbital lacrimal glands were then removed and processed for measurement of protein secretion, histology, immunohistochemistry, and Western blotting.<br />Results: The results show that lacrimal gland acinar cells are lost through programmed cell death (apoptosis) and autophagy. They also show that the number of nestin (a stem cell marker)-positive cells increased 2 to 3 days after injury and that some of these cells were also positive for Ki67 (a cell proliferation marker) and alpha-smooth muscle actin (a marker of myoepithelial cells). Finally, they show that the amount of phosphorylated Smad1/5/8 (effector molecules of bone morphogenetic protein 7 [BMP7]) increased 2 to 3 days after injury and could also be detected in nestin-positive cells.<br />Conclusions: The lacrimal gland contains stem/progenitor cells capable of tissue repair after injury. Programmed cell death after injury triggers proliferation and differentiation of these cells, presumably through activation of the BMP7 pathway.
- Subjects :
- Animals
Apoptosis drug effects
Autophagy
Blotting, Western
Cell Differentiation
Cell Proliferation
Dacryocystitis chemically induced
Dacryocystitis pathology
Disease Models, Animal
Female
Fluorescent Antibody Technique, Indirect
In Situ Nick-End Labeling
Inflammation chemically induced
Inflammation metabolism
Inflammation pathology
Interleukin-1alpha pharmacology
Intermediate Filament Proteins metabolism
Ki-67 Antigen metabolism
Lacrimal Apparatus drug effects
Lacrimal Apparatus ultrastructure
Lysosomal Membrane Proteins metabolism
Mice
Mice, Inbred BALB C
Microtubule-Associated Proteins metabolism
Nerve Tissue Proteins metabolism
Nestin
Recombinant Proteins pharmacology
Smad1 Protein metabolism
Smad5 Protein metabolism
Smad8 Protein metabolism
Stem Cells physiology
Dacryocystitis metabolism
Lacrimal Apparatus metabolism
Wound Healing physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1552-5783
- Volume :
- 49
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Investigative ophthalmology & visual science
- Publication Type :
- Academic Journal
- Accession number :
- 18586880
- Full Text :
- https://doi.org/10.1167/iovs.08-1730