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A human TRIM5alpha B30.2/SPRY domain mutant gains the ability to restrict and prematurely uncoat B-tropic murine leukemia virus.
- Source :
-
Virology [Virology] 2008 Sep 01; Vol. 378 (2), pp. 233-42. Date of Electronic Publication: 2008 Jun 30. - Publication Year :
- 2008
-
Abstract
- Human TRIM5alpha restricts N-tropic murine leukemia virus (N-MLV) but not B-tropic MLV (B-MLV) infection. Here we study B30.2/SPRY domain mutants of human TRIM5alpha that acquire the ability to inhibit B-MLV infection prior to reverse transcription without losing the ability to restrict N-MLV infection. Remarkably, these mutants gain the ability to decrease the amount of particulate B-MLV capsids in the cytosol of infected cells. In addition, these mutants gain the ability to restrict SIV(mac) and HIV-2 infection. B-MLV and SIV(mac) infections were blocked by the mutant TRIM5alpha proteins prior to reverse transcription. Thus, the range of retroviruses restricted by human TRIM5alpha can be increased by changes in the B30.2/SPRY domain, which also result in the ability to cause premature uncoating of the restricted retroviral capsid.
- Subjects :
- Antiviral Restriction Factors
Capsid metabolism
Carrier Proteins genetics
Cell Line
Cytoplasm virology
Genes, Reporter
Green Fluorescent Proteins analysis
Green Fluorescent Proteins genetics
HIV-2 immunology
Humans
Leukemia Virus, Murine physiology
Protein Structure, Tertiary
Simian Immunodeficiency Virus immunology
Tripartite Motif Proteins
Ubiquitin-Protein Ligases
Carrier Proteins physiology
Leukemia Virus, Murine immunology
Mutation, Missense
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 378
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 18586294
- Full Text :
- https://doi.org/10.1016/j.virol.2008.05.008