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Superoxide dismutase activity as a measure of hepatic oxidative stress in cattle following ethionine administration.
- Source :
-
Veterinary journal (London, England : 1997) [Vet J] 2009 Nov; Vol. 182 (2), pp. 336-41. Date of Electronic Publication: 2008 Jun 30. - Publication Year :
- 2009
-
Abstract
- The goal of this study was to assess if oxidative stress, as measured by alterations in the concentrations of antioxidant enzymes in the liver and erythrocytes of cattle, could be induced following dl-ethionine administration. Whole blood, serum and liver biopsy samples were collected 0, 4, 7 and 10 days after intra-peritoneal ethionine administration to five cows. The activities of the antioxidant enzymes copper zinc superoxide dismutase (Cu, Zn SOD) and catalase were assessed in the liver biopsies which were also examined histopathologically. Significant increases in hepatic Cu, Zn SOD concentrations (P<0.01) were noted on days 7 and 10 post-treatment. Hepatic catalase activity decreased significantly (P<0.01) on days 4, 7 and 10 post-treatment and erythrocyte Cu, Zn SOD activity was significantly increased on day 10. Serum biochemical analysis revealed a significant increase (P<0.01) in non-esterified fatty acid concentrations on day 4 and significant decreases in total cholesterol and phospholipid levels on days 4 (P<0.05), 7 (P<0.01) and 10 (P<0.01). In this model system, dl-ethionine administration was effective in inducing oxidative stress particularly reflected in the liver.
- Subjects :
- Animals
Biopsy veterinary
Catalase blood
Catalase metabolism
Cattle blood
Cholesterol blood
Fatty Acids, Nonesterified blood
Female
Histocytochemistry veterinary
Liver drug effects
Liver metabolism
Phospholipids blood
Superoxide Dismutase blood
Antimetabolites pharmacology
Cattle metabolism
Ethionine pharmacology
Liver enzymology
Oxidative Stress drug effects
Superoxide Dismutase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2971
- Volume :
- 182
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Veterinary journal (London, England : 1997)
- Publication Type :
- Academic Journal
- Accession number :
- 18585936
- Full Text :
- https://doi.org/10.1016/j.tvjl.2008.05.013