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Enhanced resistance to cattle grub infestation (Hypoderma lineatum de Vill.) in calves immunized with purified hypodermin A, B and C plus monophosphoryl lipid A (MPL).
- Source :
-
Veterinary parasitology [Vet Parasitol] 1991 Mar; Vol. 38 (2-3), pp. 185-97. - Publication Year :
- 1991
-
Abstract
- The influence of an antigen-specific cellular and humoral immune response, stimulated by immunization, on survival of a challenge infestation of Hypoderma lineatum was investigated. Calves immunized with a purified combination of hypodermin A, B and C plus monophosphoryl lipid A (MPL) developed a strong antigen-specific cellular immune response by completion of the immunization schedule which persisted to 12 weeks post-infestation. Responsiveness of peripheral blood lymphocytes to the mitogens concanavalin A and pokeweed was also elevated at 4 and 12 weeks post-infestation. Western blot analysis at the time of maximum grub counts demonstrated that immunized calves responded to hypodermin A, B and C while those receiving only MPL or infested controls responded only to hypodermin B and C. The antigen-specific antibody response as measured by ELISA at maximum grub count was significantly higher in vaccinated calves than in infested controls while the response in calves receiving only immunostimulator was also significantly elevated. Immunized (antigen plus MPL) calves produced 5.0 +/- 6.9 grubs per animal which successfully pupated while those receiving MPL alone produced 16.4 +/- 6.1 and infested controls produced 32.2 +/- 10.9 grubs per animal.
- Subjects :
- Animals
Blotting, Western veterinary
Cattle
Cattle Diseases parasitology
Diptera growth & development
Enzyme-Linked Immunosorbent Assay veterinary
Female
Hypodermyiasis immunology
Immunity, Innate immunology
Lipid A analogs & derivatives
Lipid A immunology
Antigens immunology
Cattle Diseases immunology
Diptera immunology
Hypodermyiasis veterinary
Serine Endopeptidases immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0304-4017
- Volume :
- 38
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- Veterinary parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 1858288
- Full Text :
- https://doi.org/10.1016/0304-4017(91)90128-i