Back to Search
Start Over
Distinct signature type I interferon responses are determined by the infecting virus and the target cell.
- Source :
-
Antiviral therapy [Antivir Ther] 2008; Vol. 13 (3), pp. 409-22. - Publication Year :
- 2008
-
Abstract
- Background: Type I interferons (IFN) include multiple IFN-alpha subtypes which exhibit considerable amino acid identity and activate the same cell-surface receptor. The promoter regions of the IFN-alpha genes, however, have different transcription factor binding sites, implying differential transcriptional activation. Evolutionary conservation of multiple subtypes may have resulted from external pressures associated with the crucial nature of an IFN response, namely that different viruses that are tropic for different target tissues determine the nature and extent of an IFN response, specifically the IFN-alpha subtype profile.<br />Methods: Studies were undertaken to examine inducible IFN gene expression profiles in response to infection with single-stranded RNA viruses: Sendai virus (SeV), murine hepatitis virus (MHV-1) and coxsackie virus B3 (CVB3).<br />Results: In vitro, distinct signature profiles of SeV and MHV-1-inducible gene expression for IFN-alpha2, IFN-alpha4 and IFN-alpha5 subtypes in L2 and L929 mouse fibroblast cells, in relation to the extent and kinetics of their induction, were identified. In vivo, whereas A/J mice are highly permissive for both MHV-1 and CVB3 infections and mount a poor IFN response, C57B1/6 mice are relatively resistant to both virus infections and mount a vigorous IFN response.<br />Conclusions: These data suggest that the infecting virus and the target cell type dictate the extent and signature of inducible type I IFN gene expression. The extent of IFN response to viral infection influences the subsequent biological outcome: a robust IFN response prescribes a level of resistance, whereas a poor IFN response contributes towards a permissive phenotype for infection.
- Subjects :
- Animals
Base Sequence
Cell Line
DNA Primers
Female
Interferon-alpha genetics
Kinetics
Mice
Mice, Inbred A
Mice, Inbred C57BL
Molecular Sequence Data
Polymerase Chain Reaction methods
Promoter Regions, Genetic
Species Specificity
Enterovirus B, Human pathogenicity
Fibroblasts immunology
Fibroblasts virology
Interferon-alpha metabolism
Murine hepatitis virus pathogenicity
Sendai virus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1359-6535
- Volume :
- 13
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Antiviral therapy
- Publication Type :
- Academic Journal
- Accession number :
- 18572754