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Nutrient control of release of pancreatic enzymes in yellowtail (Seriola quinqueradiata): involvement of CCK and PY in the regulatory loop.
- Source :
-
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2008 Aug; Vol. 150 (4), pp. 438-43. Date of Electronic Publication: 2008 May 09. - Publication Year :
- 2008
-
Abstract
- Cholecystokinin (CCK) and neuropeptide Y (NPY)-related peptides are key regulators of pancreatic enzyme secretion in vertebrates. CCK stimulates enzyme secretion whereas peptide Y (PY), a NPY-related peptide, plays an antagonistic role to that of CCK. In fish, very little is known about how different nutrients affect the synthesis of CCK and PY in the digestive tract, and the mechanism by which CCK and PY actually regulate digestive enzyme secretion is not well understood. In order to determine how different nutrients stimulate the synthesis of CCK and PY in yellowtail (Seriola quinqueradiata), CCK and PY mRNA levels in the digestive tract were measured after oral administration of a single bolus of either phosphate-buffered saline (PBS: control), starch (carbohydrate), casein (protein), oleic acid (fatty acid) or tri-olein (triglyceride). In addition, in order to confirm the synthesis and secretion of digestive enzymes, the mRNA levels and enzymatic activities of three digestive enzymes (lipase, trypsin and amylase) were also analyzed. Casein, oleic acid and tri-olein increased the synthesis of lipase, trypsin and amylase, while starch and PBS did not affect the activity of any of these enzymes. CCK mRNA levels rose, while PY mRNA levels were reduced in fish administered casein, oleic acid and tri-olein. These results suggest that in yellowtail, CCK and PY maintain antagonistic control of pancreatic enzyme secretion after intake of protein and/or fat.
- Subjects :
- Amylases metabolism
Animal Feed
Animals
Food
Gastrointestinal Tract enzymology
Gene Expression Profiling
Gene Expression Regulation, Enzymologic
Lipase metabolism
Models, Biological
Perciformes
RNA, Messenger metabolism
Trypsin metabolism
Cholecystokinin physiology
Neuropeptide Y physiology
Pancreas enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1531-4332
- Volume :
- 150
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
- Publication Type :
- Academic Journal
- Accession number :
- 18571447
- Full Text :
- https://doi.org/10.1016/j.cbpa.2008.05.003