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Plasmodium lipid rafts contain proteins implicated in vesicular trafficking and signalling as well as members of the PIR superfamily, potentially implicated in host immune system interactions.
- Source :
-
Proteomics [Proteomics] 2008 Jun; Vol. 8 (12), pp. 2500-13. - Publication Year :
- 2008
-
Abstract
- Plasmodium parasites, the causal agents of malaria, dramatically modify the infected erythrocyte by exporting parasite proteins into one or multiple erythrocyte compartments, the cytoplasm and the plasma membrane or beyond. Despite advances in defining signals and specific cellular compartments implicated in protein trafficking in Plasmodium-infected erythrocytes, the contribution of lipid-mediated sorting to this cellular process has been poorly investigated. In this study, we examined the proteome of cholesterol-rich membrane microdomains or lipid rafts, purified from erythrocytes infected by the rodent parasite Plasmodium berghei. Besides structural proteins associated with invasive forms, we detected chaperones, proteins implicated in vesicular trafficking, membrane fusion events and signalling. Interestingly, the raft proteome of mixed P. berghei blood stages included proteins encoded by members of a large family (bir) of putative variant antigens potentially implicated in host immune system interactions and targeted to the surface of the host erythrocytes. The generation of transgenic parasites expressing BIR/GFP fusions confirmed the dynamic association of members of this protein family with membrane microdomains. Our results indicated that lipid rafts in Plasmodium-infected erythrocytes might constitute a route to sort and fold parasite proteins directed to various host cell compartments including the cell surface.
- Subjects :
- Animals
Antigens genetics
Erythrocytes metabolism
Erythrocytes parasitology
Host-Parasite Interactions
Humans
Life Cycle Stages
Mice
Mice, Inbred BALB C
Mice, Inbred Strains
Plasmodium chemistry
Plasmodium genetics
Plasmodium metabolism
Plasmodium berghei genetics
Plasmodium berghei growth & development
Plasmodium berghei parasitology
Protein Transport
Proteomics methods
Protozoan Proteins analysis
Trypsin pharmacology
Antigens immunology
Membrane Microdomains chemistry
Plasmodium physiology
Protozoan Proteins metabolism
Signal Transduction
Transport Vesicles metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1615-9861
- Volume :
- 8
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 18563749
- Full Text :
- https://doi.org/10.1002/pmic.200700763