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Identification, cloning and characterization of a GDSL lipase secreted into the nectar of Jacaranda mimosifolia.
- Source :
-
Plant molecular biology [Plant Mol Biol] 2008 Sep; Vol. 68 (1-2), pp. 173-83. Date of Electronic Publication: 2008 Jun 13. - Publication Year :
- 2008
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Abstract
- The presence and function of several proteins secreted into floral nectars has been described in recent years. Here we report the presence of at least eight distinct proteins secreted into the floral nectar of the tropical tree Jacaranda mimosifolia (Bignoniaceae). Steps were initiated to identify and characterize these proteins in order to determine potential functions. The N-terminal sequence of the major Jacaranda nectar protein, JNP1, at 43 kDa contained similarity with members of the plant GDSL lipase/esterase gene family. Based upon this sequence, a full-length cDNA was isolated and predicted to encode a mature protein of 339 amino acids with a molecular mass of 37 kDa. Both raw nectar and heterologously expressed JNP1 displayed lipase/esterase activities. Interestingly, J. mimosifolia flowers produce an opaque, white colored nectar containing spherical, lipophilic particles approximately 5 microm in diameter and smaller. GS-MS analysis also identified the accumulation of free fatty acids within the nectar. It is proposed that JNP1 hydrolyzes Jacaranda nectar lipids with the concomitant release of free fatty acids. Potential functions of JNP1 in relation to pollinator attraction and prevention of microbial growth within nectar are briefly discussed.
- Subjects :
- Amino Acid Sequence
Base Sequence
Bignoniaceae genetics
Blotting, Western
Carboxylic Ester Hydrolases genetics
Electrophoresis, Polyacrylamide Gel
Fatty Acids metabolism
Flowers genetics
Gas Chromatography-Mass Spectrometry
Molecular Sequence Data
Plant Proteins genetics
Sequence Alignment
Sequence Homology, Amino Acid
Bignoniaceae enzymology
Carboxylic Ester Hydrolases metabolism
Flowers enzymology
Plant Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0167-4412
- Volume :
- 68
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Plant molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 18553138
- Full Text :
- https://doi.org/10.1007/s11103-008-9361-1