Back to Search
Start Over
Assays for ribosomal RNA processing and ribosome assembly.
- Source :
-
Current protocols in cell biology [Curr Protoc Cell Biol] 2008 Jun; Vol. Chapter 22, pp. Unit 22.11. - Publication Year :
- 2008
-
Abstract
- The synthesis of ribosomes is a major metabolic activity critical for cell growth and homeostasis. Understanding the mechanisms of ribosome biogenesis has important implications for studying both protein synthesis and cell cycle control. This unit describes several techniques for the analysis of rRNA maturation and ribosome assembly adapted for mammalian cells. Metabolic labeling of rRNA and hybridization analysis of precursors can be used to assess changes in rRNA processing that occur under experimental conditions of interest. Separation of preribosomal particles by sucrose gradient centrifugation is suitable for the analysis of proteins associated with preribosomes during their assembly and maturation in the cell nucleus.<br /> (Copyright 2008 by John Wiley & Sons, Inc.)
- Subjects :
- Animals
Cell Nucleolus chemistry
Cell Nucleolus metabolism
Centrifugation, Density Gradient
DNA Probes
Humans
Mammals metabolism
RNA, Ribosomal analysis
Radioisotopes analysis
Radioisotopes metabolism
Ribonucleoproteins analysis
Ribonucleoproteins metabolism
Ribosomes chemistry
Cytological Techniques
RNA Processing, Post-Transcriptional
RNA, Ribosomal metabolism
Ribosomes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1934-2616
- Volume :
- Chapter 22
- Database :
- MEDLINE
- Journal :
- Current protocols in cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 18551418
- Full Text :
- https://doi.org/10.1002/0471143030.cb2211s39