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A CK19(CreERT) knockin mouse line allows for conditional DNA recombination in epithelial cells in multiple endodermal organs.
- Source :
-
Genesis (New York, N.Y. : 2000) [Genesis] 2008 Jun; Vol. 46 (6), pp. 318-23. - Publication Year :
- 2008
-
Abstract
- Cre/LoxP-mediated DNA recombination allows for gene function and cell lineage analyses during embryonic development and tissue regeneration. Here, we describe the derivation of a K19(CreERT) mouse line in which the tamoxifen-activable CreER(T) was knocked into the endogenous cytokeratin 19 locus. In the absence of tamoxifen, leaky Cre activity could be detected only in less than 1% of stomach and intestinal epithelial cells, but not in pancreatic or hepatic epithelial tissues. Tamoxifen administration in postnatal animals induced widespread DNA recombination in epithelial cells of pancreatic ducts, hepatic ducts, stomach, and intestine in a dose-dependent manner. Significantly, we found that Cre activity could be induced in the putative gut stem/progenitor cells that sustained long-term gut epithelial expression of a Cre reporter. This mouse line should therefore provide a valuable reagent for manipulating gene activity and for cell lineage marking in multiorgans during normal tissue homeostasis and regeneration.<br /> ((c) 2008 Wiley-Liss, Inc.)
- Subjects :
- Alleles
Animals
Cell Lineage
Dose-Response Relationship, Drug
Epithelial Cells enzymology
Gene Targeting methods
Genes, Reporter
Immunohistochemistry
Integrases metabolism
Intestine, Small enzymology
Keratin-19 metabolism
Mice
Mice, Inbred C57BL
Mice, Transgenic
Organ Specificity genetics
Stem Cells enzymology
Stem Cells metabolism
Tamoxifen pharmacology
DNA genetics
Epithelial Cells metabolism
Intestine, Small metabolism
Keratin-19 genetics
Recombination, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1526-968X
- Volume :
- 46
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Genesis (New York, N.Y. : 2000)
- Publication Type :
- Academic Journal
- Accession number :
- 18543299
- Full Text :
- https://doi.org/10.1002/dvg.20397