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Hoechst 33258--poly(dG-dC).poly(dG-dC) complexes of three types.
- Source :
-
Journal of biomolecular structure & dynamics [J Biomol Struct Dyn] 2008 Aug; Vol. 26 (1), pp. 99-114. - Publication Year :
- 2008
-
Abstract
- It was found recently that Hoechst 33258, a dsDNA fluorescent dye used in cytological studies, is an efficient inhibitor of the interaction of TATA-box-binding protein with DNA, DNA topoisomerase I, and DNA helicases. In addition it proved to be a radioprotector. Biological activity of Hoechst 33258 may be associated with dsDNA complexes of not only monomeric, but also dimeric type. In this work, the Hoechst 33258 interaction with poly(dG-dC).poly(dG-dC) was studied using UV-vis and fluorescent spectroscopy, circular and flow-type linear dichroism. It was found that Hoechst 33258 formed with poly(dG-dC).poly(dG-dC) complexes of three types, namely, monomeric, dimeric, and, apparently, tetrameric, and their spectral properties were studied. Complexes of monomeric and dimeric types competed with distamycin A, a minor groove ligand, for binding to poly(dG-dC).poly(dG-dC). We proposed that Hoechst 33258 both monomers and dimers form complexes of the external type with poly(dG-dC).poly(dG-dC) from the side of the minor groove.
- Subjects :
- Circular Dichroism
Dimerization
Models, Molecular
Nucleic Acid Conformation
Poly C chemistry
Poly G chemistry
Polydeoxyribonucleotides
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Bisbenzimidazole metabolism
DNA Topoisomerases, Type I metabolism
Fluorescent Dyes metabolism
Poly C metabolism
Poly G metabolism
Polynucleotides metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0739-1102
- Volume :
- 26
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of biomolecular structure & dynamics
- Publication Type :
- Academic Journal
- Accession number :
- 18533731
- Full Text :
- https://doi.org/10.1080/07391102.2008.10507228