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Patterns of angiogenic and hematopoietic gene expression during brown trout embryogenesis.
Patterns of angiogenic and hematopoietic gene expression during brown trout embryogenesis.
- Source :
-
Journal of experimental zoology. Part B, Molecular and developmental evolution [J Exp Zool B Mol Dev Evol] 2008 Sep 15; Vol. 310 (6), pp. 479-91. - Publication Year :
- 2008
-
Abstract
- In this article, whole mount in situ hybridization is used to examine early blood vessel and blood cell development in the embryos of the brown trout Salmo trutta lacustris. cDNAs encoding for the angiogenic markers fli1 and flk1, and for the hematopoietic markers gata1 and gata2, were identified from an expressed sequence tag library of rainbow trout. Results show that fli1, flk1 and gata2 are activated in bilateral bands of the lateral trunk mesoderm before the onset of somitogenesis, shortly followed by gata1. These bands then converge toward the ventral midline to form the intermediate cell mass (ICM) (anterior ICM). Subsequent axial vasculogenesis and initial blood cell formation involve a clear spatial separation of fli1 and gata gene expression. Fli1 staining is most intense within the axial vessel (dorsal aorta, posterior cardinal vein) forming and lateral ICM cells, whereas binding of gata1 and gata2 probes becomes confined to the central portion of ICM cells beneath the dorsal aorta. This is followed by a first wave of angiogenesis, indicated by expression of fli1 and flk1. This gives rise to the intersegmental, dorsal longitudinal anastomotic and intestinal vessels. Further angiogenesis and hematopoiesis are activated in the "posterior ICM" of the tail. Here, the absence of gata1 indicates that hematopoiesis in this tissue generates myeloid rather than erythroid cells. The results supplement and validate previous, now historical morphological work in salmonids, thus aiding the elucidation of a comprehensive general scheme of angiogenic and hematopoietic development in the teleost embryo.
- Subjects :
- Amino Acid Sequence
Animals
Blood Vessels metabolism
GATA1 Transcription Factor genetics
GATA1 Transcription Factor metabolism
GATA2 Transcription Factor genetics
GATA2 Transcription Factor metabolism
Hematopoietic System metabolism
In Situ Hybridization
Molecular Sequence Data
Proto-Oncogene Protein c-fli-1 genetics
Proto-Oncogene Protein c-fli-1 metabolism
Vascular Endothelial Growth Factor Receptor-1 genetics
Vascular Endothelial Growth Factor Receptor-1 metabolism
Zebrafish genetics
Blood Vessels embryology
Embryonic Development genetics
Gene Expression Regulation, Developmental
Hematopoietic System embryology
Neovascularization, Physiologic genetics
Trout embryology
Subjects
Details
- Language :
- English
- ISSN :
- 1552-5015
- Volume :
- 310
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of experimental zoology. Part B, Molecular and developmental evolution
- Publication Type :
- Academic Journal
- Accession number :
- 18512703
- Full Text :
- https://doi.org/10.1002/jez.b.21220