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DNA topoisomerase II from mammalian mitochondria is inhibited by the antitumor drugs, m-AMSA and VM-26.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1991 Apr 30; Vol. 176 (2), pp. 690-7. - Publication Year :
- 1991
-
Abstract
- A type II DNA topoisomerase has been partially purified from calf thymus mitochondria by a combination of differential centrifugation and column chromatography. The mitochondrial enzyme was inhibited by amsacrine (m-AMSA) slightly at 0.5 microM, significantly at 5.0 microM, and completely at 50 microM. A similar profile was obtained with teniposide (VM-26) although the latter drug was not quite as potent an inhibitor as the former. P4 unknotting assays of the purified nuclear type II topoisomerase in the presence of m-AMSA and VM-26 indicated that the mitochondrial and nuclear enzymes behaved similarly, although the mitochondrial enzyme appeared to be inhibited more strongly.
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 176
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 1851000
- Full Text :
- https://doi.org/10.1016/s0006-291x(05)80239-6