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Quantitative expression analysis of selected COR genes reveals their differential expression in leaf and crown tissues of wheat (Triticum aestivum L.) during an extended low temperature acclimation regimen.
- Source :
-
Journal of experimental botany [J Exp Bot] 2008; Vol. 59 (9), pp. 2393-402. Date of Electronic Publication: 2008 May 28. - Publication Year :
- 2008
-
Abstract
- A number of COR genes (COld-Regulated genes) have been implicated in the acquisition of low temperature (LT) tolerance in wheat (Triticum aestivum L.). This study compared the relative expression patterns of selected COR genes in leaf and crown tissues of wheat near-isogenic lines to increase understanding of the molecular mechanisms underlying LT acclimation. Reciprocal near-isogenic lines were generated such that the dominant Vrn-A1 and recessive vrn-A1 loci were interchanged in a spring cv. Manitou and a winter cv. Norstar. Phenological development, acquisition of LT tolerance, and WCS120 polypeptide accumulation in these genotypes proceeded at rates similar to those previously reported for 6 degrees C acclimation from 0 to 98 d. However, a differential accumulation of WCS120 polypeptide and expression of the COR genes Wcs120, Wcor410, and Wcor14 was observed in the leaf and crown tissues. COR gene transcript levels peaked at 2 d of the acclimation period in both tissues and differences among genotypes were most evident at this time. COR gene expression was highest for the LT-tolerant and lowest for the tender genotypes. However, expression rates were divergent enough in genotypes with intermediate hardiness that comparisons among tissues and/or times during acclimation often resulted in variable interpretations of the relative expression of the COR genes in the determination of LT tolerance. These observations emphasize the need to pay close attention to experimental conditions, sampling times, and genotype and tissue selection in experiments designed to identify the critical genetic components that interact to determine LT acclimation.
- Subjects :
- Cold Temperature
DNA-Binding Proteins metabolism
Flowering Tops genetics
Gene Expression
Heat-Shock Proteins metabolism
Plant Leaves genetics
Plant Proteins metabolism
Seasons
Triticum genetics
Acclimatization
DNA-Binding Proteins genetics
Flowering Tops physiology
Gene Expression Regulation, Plant
Heat-Shock Proteins genetics
Plant Leaves physiology
Plant Proteins genetics
Triticum physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2431
- Volume :
- 59
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of experimental botany
- Publication Type :
- Academic Journal
- Accession number :
- 18508811
- Full Text :
- https://doi.org/10.1093/jxb/ern112