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Hormonal regulation of regucalcin mRNA expression in osteoblastic MC3T3-E1 cells.
- Source :
-
International journal of molecular medicine [Int J Mol Med] 2008 Jun; Vol. 21 (6), pp. 771-5. - Publication Year :
- 2008
-
Abstract
- The effect of various hormones on regucalcin mRNA expression in osteoblastic MC3T3-E1 cells in vitro was investigated. Cells with subconfluency were cultured for 24 or 48 h in a medium containing either vehicle or various hormones without fetal bovine serum. Regucalcin mRNA expression was significantly increased after culture with parathyroid hormone (synthetic human PTH; 10(-7) M), insulin-like growth factor-I (IGF-I; 10(-8) M), or 17beta-estradiol (10(-10) or 10(-9) M) for 48 h. Culture with 1,25-dihydroxyvitamin D3 (10(-7) M) for 48 h caused a significant decrease in regucalcin mRNA expression. Regucalcin mRNA expression was significantly decreased after culture with tumor necrosis factor-alpha (1 or 10 ng/ml of medium) for 24 or 48 h. The effect of PTH or IGF-I in increasing regucalcin mRNA expression was not seen in the presence of staurosporine (10(-8) M), an inhibitor of protein kinase C, or PD98059 (10(-7) M), an inhibitor of mitosis-activated protein kinase (MAP kinase), respectively, suggesting that regucalcin mRNA expression is enhanced through intracellular signaling factors. This study demonstrated that regucalcin mRNA expression in osteoblastic MC3T3-E1 cells is regulated by various hormones.
- Subjects :
- 3T3 Cells
Animals
Calcitriol pharmacology
Cell Differentiation drug effects
Cell Differentiation genetics
Dose-Response Relationship, Drug
Estradiol pharmacology
Flavonoids pharmacology
Humans
Insulin-Like Growth Factor I pharmacology
Mice
Osteoblasts cytology
Osteoblasts metabolism
Parathyroid Hormone pharmacology
RNA, Messenger metabolism
Reverse Transcriptase Polymerase Chain Reaction methods
Signal Transduction drug effects
Signal Transduction genetics
Staurosporine pharmacology
Tumor Necrosis Factor-alpha pharmacology
Calcium-Binding Proteins genetics
Hormones pharmacology
Intracellular Signaling Peptides and Proteins genetics
Osteoblasts drug effects
RNA, Messenger genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1107-3756
- Volume :
- 21
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- International journal of molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 18506371