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ChlaDub1 of Chlamydia trachomatis suppresses NF-kappaB activation and inhibits IkappaBalpha ubiquitination and degradation.
- Source :
-
Cellular microbiology [Cell Microbiol] 2008 Sep; Vol. 10 (9), pp. 1879-92. Date of Electronic Publication: 2008 Jun 28. - Publication Year :
- 2008
-
Abstract
- Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes various human diseases, including blindness caused by ocular infection and sexually transmitted diseases resulting from urogenital infection. After infecting host cells, Chlamydiae avoid alarming the host's immune system. Among the immune evasion mechanisms, Chlamydiae can inhibit NF-kappaB activation, a crucial pathway for host inflammatory responses. In this study, we show that ChlaDub1, a deubiquitinating and deNeddylating protease from C. trachomatis, is expressed in infected cells. In transfection experiments, ChlaDub1 suppresses NF-kappaB activation induced by several pro-inflammatory stimuli and binds the NF-kappaB inhibitory subunit IkappaBalpha, impairing its ubiquitination and degradation. Thus, we provide further insight into the mechanism by which C. trachomatis may evade the host inflammatory response by demonstrating that ChlaDub1, a protease produced by this microorganism, is capable of inhibiting IkappaBalpha degradation and blocking NF-kappaB activation.
- Subjects :
- Bacterial Proteins genetics
Cell Line
Chlamydia Infections microbiology
Down-Regulation
Endopeptidases genetics
Humans
NF-KappaB Inhibitor alpha
Protein Binding
Signal Transduction
TNF Receptor-Associated Factor 2 metabolism
TNF Receptor-Associated Factor 6 metabolism
Transfection
Ubiquitin-Specific Proteases
Ubiquitination
Bacterial Proteins metabolism
Chlamydia Infections metabolism
Chlamydia trachomatis enzymology
Endopeptidases metabolism
I-kappa B Proteins metabolism
NF-kappa B antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 1462-5822
- Volume :
- 10
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Cellular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 18503636
- Full Text :
- https://doi.org/10.1111/j.1462-5822.2008.01178.x