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Saccharomyces cerevisiae Grx6 and Grx7 are monothiol glutaredoxins associated with the early secretory pathway.
- Source :
-
Eukaryotic cell [Eukaryot Cell] 2008 Aug; Vol. 7 (8), pp. 1415-26. Date of Electronic Publication: 2008 May 23. - Publication Year :
- 2008
-
Abstract
- Saccharomyces cerevisiae Grx6 and Grx7 are two monothiol glutaredoxins whose active-site sequences (CSYS and CPYS, respectively) are reminiscent of the CPYC active-site sequence of classical dithiol glutaredoxins. Both proteins contain an N-terminal transmembrane domain which is responsible for their association to membranes of the early secretory pathway vesicles, facing the luminal side. Thus, Grx6 localizes at the endoplasmic reticulum and Golgi compartments, while Grx7 is mostly at the Golgi. Expression of GRX6 is modestly upregulated by several stresses (calcium, sodium, and peroxides) in a manner dependent on the Crz1-calcineurin pathway. Some of these stresses also upregulate GRX7 expression under the control of the Msn2/4 transcription factor. The N glycosylation inhibitor tunicamycin induces the expression of both genes along with protein accumulation. Mutants lacking both glutaredoxins display reduced sensitivity to tunicamycin, although the drug is still able to manifest its inhibitory effect on a reporter glycoprotein. Grx6 and Grx7 have measurable oxidoreductase activity in vivo, which is increased in the presence of tunicamycin. Both glutaredoxins could be responsible for the regulation of the sulfhydryl oxidative state at the oxidant conditions of the early secretory pathway vesicles. However, the differences in location and expression responses against stresses suggest that their functions are not totally overlapping.
- Subjects :
- DNA-Binding Proteins metabolism
Endoplasmic Reticulum metabolism
Endoplasmic Reticulum ultrastructure
Enzyme Inhibitors pharmacology
Gene Expression Regulation, Fungal drug effects
Gene Expression Regulation, Fungal physiology
Glutaredoxins genetics
Glycosylation drug effects
Golgi Apparatus metabolism
Golgi Apparatus ultrastructure
Oxidoreductases Acting on Sulfur Group Donors metabolism
Saccharomyces cerevisiae Proteins genetics
Secretory Vesicles genetics
Secretory Vesicles metabolism
Secretory Vesicles ultrastructure
Transcription Factors metabolism
Glutaredoxins metabolism
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins metabolism
Signal Transduction genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1535-9786
- Volume :
- 7
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Eukaryotic cell
- Publication Type :
- Academic Journal
- Accession number :
- 18503006
- Full Text :
- https://doi.org/10.1128/EC.00133-08