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Eukaryotic elongation factor 1A interacts with Turnip mosaic virus RNA-dependent RNA polymerase and VPg-Pro in virus-induced vesicles.

Authors :
Thivierge K
Cotton S
Dufresne PJ
Mathieu I
Beauchemin C
Ide C
Fortin MG
Laliberté JF
Source :
Virology [Virology] 2008 Jul 20; Vol. 377 (1), pp. 216-25. Date of Electronic Publication: 2008 May 23.
Publication Year :
2008

Abstract

Eukaryotic elongation factor 1-alpha (eEF1A) was identified as an interactor of Turnip mosaic virus (TuMV) RNA-dependent RNA polymerase (RdRp) and VPg-protease (VPg-Pro) using tandem affinity purification and/or in vitro assays. Subcellular fractionation experiments revealed that the level of eEF1A substantially increased in membrane fractions upon TuMV infection. Replication of TuMV occurs in cytoplasmic membrane vesicles, which are induced by 6K-VPg-Pro. Confocal microscopy indicated that eEF1A was included in these vesicles. To confirm that eEF1A was found in replication vesicles, we constructed an infectious recombinant TuMV that contains an additional copy of the 6K protein fused to the green fluorescent protein (GFP). In cells infected with this recombinant TuMV, fluorescence emitted by 6KGFP was associated with cytoplasmic membrane vesicles that contained VPg-Pro, the eukaryotic initiation factor (iso) 4E, the poly(A)-binding protein, the heat shock cognate 70-3 protein, and eEF1A. These results suggest that TuMV-induced membrane vesicles host at least three plant translation factors in addition to the viral replication proteins.

Details

Language :
English
ISSN :
0042-6822
Volume :
377
Issue :
1
Database :
MEDLINE
Journal :
Virology
Publication Type :
Academic Journal
Accession number :
18501944
Full Text :
https://doi.org/10.1016/j.virol.2008.04.015