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Monitoring of Leuconostoc mesenteroides DRC starter in fermented vegetable by random integration of chloramphenicol acetyltransferase gene.
- Source :
-
Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2008 Sep; Vol. 35 (9), pp. 953-9. Date of Electronic Publication: 2008 May 24. - Publication Year :
- 2008
-
Abstract
- In 2004, Leuconostoc mesenteroides DRC was first used as a starter culture for achieving higher organoleptic effects in Korean kimchi manufacture. For a better understanding of starter growth in a mixed culture system, and for predicting starter predominance in kimchi, a monitoring system for the starter was established. The chloramphenicol resistance marker gene (cat) was randomly integrated into chromosomal DNA of L. mesenteroides DRC using a viral transposon and transposase. The DRC mutant, tDRC2, had a similar growth pattern to the host strain, with no major alteration in phenotypic characteristics. The mutant strain was inoculated into real kimchi, and monitoring of the starter population was successfully achieved. The overall predominance of Leuconostoc in kimchi inoculated with DRC followed the general growth pattern of this genus during kimchi fermentation. Our results also demonstrate the competitive ability of the DRC starter against Leuconostoc from natural flora, maintaining its predominance above 88% during the whole fermentation period. Based on this experiment, the random gene integration method using a transposon was shown to be of utility in transferring any commercial starter into a selectable and monitorable strain for simulation purposes.
- Subjects :
- Amino Acid Sequence
Base Sequence
Chloramphenicol O-Acetyltransferase chemistry
Chloramphenicol O-Acetyltransferase metabolism
Chromosomes, Bacterial genetics
DNA Transposable Elements genetics
Food Microbiology
Leuconostoc growth & development
Molecular Sequence Data
Time Factors
Brassica metabolism
Chloramphenicol O-Acetyltransferase genetics
Fermentation
Food Analysis methods
Leuconostoc enzymology
Leuconostoc genetics
Mutagenesis, Insertional genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1367-5435
- Volume :
- 35
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of industrial microbiology & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 18500545
- Full Text :
- https://doi.org/10.1007/s10295-008-0369-y