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Photodynamic treatment of endodontic polymicrobial infection in vitro.
- Source :
-
Journal of endodontics [J Endod] 2008 Jun; Vol. 34 (6), pp. 728-34. Date of Electronic Publication: 2008 Apr 25. - Publication Year :
- 2008
-
Abstract
- We investigated the photodynamic effects of methylene blue on multispecies root canal biofilms comprising Actinomyces israelii, Fusobacterium nucleatum subspecies nucleatum, Porphyromonas gingivalis, and Prevotella intermedia in experimentally infected root canals of extracted human teeth in vitro. The 4 test microorganisms were detected in root canals by using DNA probes. Scanning electron microscopy showed the presence of biofilms in root canals before therapy. Root canal systems were incubated with methylene blue (25 microg/mL) for 10 minutes followed by exposure to red light at 665 nm with an energy fluence of 30 J/cm(2). Light was delivered from a diode laser via a 250-microm diameter polymethyl methacrylate optical fiber that uniformly distributed light over 360 degrees. Photodynamic therapy (PDT) achieved up to 80% reduction of colony-forming unit counts. We concluded that PDT can be an effective adjunct to standard endodontic antimicrobial treatment when the PDT parameters are optimized.
- Subjects :
- Bacteria, Anaerobic drug effects
Biofilms drug effects
Colony Count, Microbial
DNA, Bacterial analysis
Dental Pulp Necrosis drug therapy
Dental Pulp Necrosis microbiology
Humans
Linear Models
Methylene Blue pharmacology
Microscopy, Confocal
Microscopy, Electron, Scanning
Bacterial Infections drug therapy
Dental Pulp Cavity microbiology
Enzyme Inhibitors therapeutic use
Lasers, Semiconductor therapeutic use
Methylene Blue therapeutic use
Photochemotherapy
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3554
- Volume :
- 34
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of endodontics
- Publication Type :
- Academic Journal
- Accession number :
- 18498901
- Full Text :
- https://doi.org/10.1016/j.joen.2008.03.011