Back to Search
Start Over
Elevated serum anti-Saccharomyces cerevisiae, anti-I2 and anti-OmpW antibody levels in patients with suspicion of celiac disease.
- Source :
-
Journal of clinical immunology [J Clin Immunol] 2008 Sep; Vol. 28 (5), pp. 486-94. Date of Electronic Publication: 2008 May 22. - Publication Year :
- 2008
-
Abstract
- Objectives: Expression of anti-Saccharomyces cerevisiae antibodies (ASCA) identifies patients and individuals at risk for Crohn's disease and has also been reported in 40-60% of celiac disease (CD) cases, suggesting a role of host response to enteric microbiota in the development of inflammatory lesions. In this prospective study in patients with suspicion of CD, we evaluate the frequency and association of ASCA to serological responses for other host microbial targets formally associated with Crohn's disease, including the Pseudomonas fluorescens associated sequence I2 and a Bacteroides caccae TonB-linked outer membrane protein, OmpW.<br />Methods: Small bowel mucosal biopsies were taken from 242 patients with suspicion of CD, their sera were tested for antibodies to tissue transglutaminase (tTG), ASCA, I2, and OmpW. Eighty adult healthy blood donors were used as controls.<br />Results: The diagnosis of CD was confirmed on biopsy in 134 cases. The occurrence of ASCA and I2 positivity was significantly higher in adult CD patients than in patients with non-CD disease. Anti-I2 levels in the sera were significantly higher in adult CD patients than in non-CD disease or the controls and anti-OmpW levels in CD and non-CD patients when compared to controls. Positive seroreactivity to OmpW seemed to increase with age. Of the CD patients, 90% were seropositive for at least one microbial antigen tested.<br />Conclusions: This study demonstrates a mosaic of disease-related serological responses to microbial antigens in patients with CD. Immune responses to commensal enteric bacteria may play a role in the small intestine mucosal damage in CD.
- Subjects :
- Adolescent
Adult
Aged
Bacterial Outer Membrane Proteins immunology
Bacterial Proteins immunology
Bacterial Proteins metabolism
Biomarkers
Celiac Disease blood
Celiac Disease diagnosis
Celiac Disease microbiology
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Infant
Male
Middle Aged
Prospective Studies
Pseudomonas fluorescens immunology
Saccharomyces cerevisiae Proteins immunology
Serologic Tests
Antibodies, Heterophile blood
Bacterial Outer Membrane Proteins metabolism
Celiac Disease immunology
Saccharomyces cerevisiae immunology
Saccharomyces cerevisiae Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0271-9142
- Volume :
- 28
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of clinical immunology
- Publication Type :
- Academic Journal
- Accession number :
- 18496744
- Full Text :
- https://doi.org/10.1007/s10875-008-9200-9