Back to Search
Start Over
Effects of Dyract AP and released ionic products on periodontal ligament cells and bone marrow cultures.
- Source :
-
Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2008 Dec; Vol. 24 (12), pp. 1623-30. Date of Electronic Publication: 2008 May 08. - Publication Year :
- 2008
-
Abstract
- Objectives: The aim of this work was to investigate the release of inorganic ionic products from specimens of the polyacid-modified composite resin Dyract AP (DAP) and furthermore, to analyze the biological effect of DAP and the medium extract in human periodontal ligament (PDL) cells and mouse bone marrow cell (BMC) cultures.<br />Methods: Ion release from DAP specimens immersed in cell culture medium was analyzed with inductively coupled plasma optical emission spectroscopy (ICP-OES). Cells were cultured with either DAP specimens or with DAP media extract and effects on cell proliferation, osteoblastic gene expression and mineralization capacity were analyzed with direct-contact tests, neutral red (NR) uptake, quantitative real-time PCR and a bone nodule formation assay.<br />Results: ICP-OES analysis of DAP extract demonstrated a significant increase in fluoride, strontium and silica. PDL cells demonstrated normal growth pattern in the direct-contact tests with the material. DAP extracts produced a dose-dependent stimulation of cell proliferation and concomitant inhibition of osteoblast specific markers and nodule formation.<br />Significance: The compomer may have possible bioactive properties due to ions leaching out from the filler component.
- Subjects :
- Animals
Calcification, Physiologic drug effects
Cell Proliferation drug effects
Cells, Cultured
Fluorine analysis
Humans
Ions analysis
Materials Testing
Mice
Osteoblasts metabolism
Periodontal Ligament cytology
Spectrophotometry methods
Strontium analysis
Bone Marrow Cells drug effects
Compomers pharmacology
Osteogenesis drug effects
Periodontal Ligament drug effects
Root Canal Filling Materials pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0109-5641
- Volume :
- 24
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Dental materials : official publication of the Academy of Dental Materials
- Publication Type :
- Academic Journal
- Accession number :
- 18471872
- Full Text :
- https://doi.org/10.1016/j.dental.2008.03.024