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Expression of Osterix in mechanical stress-induced osteogenic differentiation of periodontal ligament cells in vitro.
- Source :
-
European journal of oral sciences [Eur J Oral Sci] 2008 Jun; Vol. 116 (3), pp. 199-206. - Publication Year :
- 2008
-
Abstract
- Osterix (Osx) is an osteoblast-specific transcription factor required for the differentiation of pre-osteoblasts into functional osteoblasts. This study sought to examine the changes of Osx expression in periodontal ligament cells (PDLC) subjected to mechanical force, and to investigate whether Osx is involved in the mechanical stress-induced differentiation of PDLC. Human PDLC were exposed to centrifugal force for 1-12 h. Real-time polymerase chain reaction (PCR), western blot, and immunofluorescence assays were used to examine the mRNA and protein expression of Osx and its subcellular localization. Furthermore, PDLC were transfected with the expression vector pcDNA3.1 flag-Osx and subjected to mechanical force for 6 h. The changes in alkaline phosphatase (ALP) activity and in the expression of core-binding factor alpha1 (Cbfa1), ALP, osteopontin, bone sialoprotein, osteocalcin, and collagen I were measured. After the application of mechanical force, Osx was upregulated in a time-dependent manner at both mRNA and protein levels, and Osx protein was translocated from the cytosol into the cell nuclei. Overexpression of Osx did not affect the expression of Cbfa1, but it significantly enhanced the ALP activity and the mRNA expression of all the aforementioned osteogenic marker genes, all of which increased further under mechanical stress. These results suggest that Osx might play an important role in the mechanical stress-induced osteogenic differentiation of PDLC and therefore be involved in alveolar bone remodeling during orthodontic therapy.
- Subjects :
- Active Transport, Cell Nucleus
Alkaline Phosphatase biosynthesis
Blotting, Western
Cell Differentiation
Cells, Cultured
Collagen Type I biosynthesis
Core Binding Factor Alpha 1 Subunit biosynthesis
Dental Stress Analysis
Fluorescent Antibody Technique
Gene Expression Profiling
Humans
Integrin-Binding Sialoprotein
Osteoblasts metabolism
Osteocalcin biosynthesis
Osteopontin biosynthesis
Polymerase Chain Reaction
Sialoglycoproteins biosynthesis
Sp7 Transcription Factor
Stress, Mechanical
Transfection
Up-Regulation
Bone Remodeling physiology
Osteoblasts cytology
Periodontal Ligament cytology
Transcription Factors biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0722
- Volume :
- 116
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- European journal of oral sciences
- Publication Type :
- Academic Journal
- Accession number :
- 18471237
- Full Text :
- https://doi.org/10.1111/j.1600-0722.2008.00533.x