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Stem cell mobilization in idiopathic steroid-sensitive nephrotic syndrome.
- Source :
-
Pediatric nephrology (Berlin, Germany) [Pediatr Nephrol] 2008 Aug; Vol. 23 (8), pp. 1251-6. Date of Electronic Publication: 2008 May 06. - Publication Year :
- 2008
-
Abstract
- Steroid-sensitive nephrotic syndrome (SSNS) is classically thought to be a T-cell disorder. The aim of this study was to examine whether or not thymus homeostasis was affected in SSNS. Mature and naive T cell recent thymic emigrants were quantified in the peripheral blood of nephrotic patients and controls. Because the generation of new T cells by the thymus ultimately depends on hematopoietic stem cells, CD34+ cells were also included in the study. Nineteen patients with SSNS during relapse, 13 with SSNS during proteinuria remission, and 18 controls were studied. Cell-surface markers (CD3, CD4, CD8, CD19, CD16, CD56, CD45RA, CD62L, CD34, and CD38) were analyzed by flow cytometric analysis. T-cell rearrangement excision circles (TRECs) were quantified in CD2+ cells by real-time polymerase chain reaction. Stroma cell-derived factor-1 (SDF-1) genotype and metalloproteinase-9 (MMP-9) plasma levels were also determined. Mature T cells (CD4+ and CD8+), circulating naive T cells (CD62L+ and CD3+ CD62L+), and recent thymic emigrants (CD45RA+) as well as TRECs, that measure thymus production, had a similar level in the three groups of patients. Conversely, CD34+ hematopoietic stem cells displayed a two-fold increase in SSNS patients during relapse either compared with controls or SSNS patients at remission. In addition, compared with controls, SSNS patients at remission displayed (1) a decrease in CD19+ cells (B cells) and (2) an increase in CD16CD56+ cells [natural killer (NK) cells]. In conclusion, thymus homeostasis is not significantly affected in nephrotic patients. Hematopoietic stem-cell mobilization at proteinuria relapse, as well as changes in B and NK cells during remission, suggest that SSNS might be due to a general disturbance of hematopoietic and immune cell trafficking.
- Subjects :
- B-Lymphocytes cytology
B-Lymphocytes immunology
Child
Female
Flow Cytometry
Humans
Killer Cells, Natural cytology
Killer Cells, Natural immunology
Male
Nephrotic Syndrome drug therapy
Proteinuria drug therapy
Proteinuria immunology
Recurrence
Remission Induction
Steroids therapeutic use
Thymus Gland cytology
Hematopoietic Stem Cells cytology
Hematopoietic Stem Cells immunology
Nephrotic Syndrome immunology
T-Lymphocytes cytology
T-Lymphocytes immunology
Thymus Gland immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0931-041X
- Volume :
- 23
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Pediatric nephrology (Berlin, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 18458957
- Full Text :
- https://doi.org/10.1007/s00467-008-0793-2