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A novel B cell-mediated transport of IgE-immune complexes to the follicle of the spleen.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2008 May 15; Vol. 180 (10), pp. 6604-10. - Publication Year :
- 2008
-
Abstract
- Ag administered i.v. to mice along with specific IgE or IgG2a induces higher Ab- and CD4(+) T cell responses than Ag administered alone. The IgE effect is completely dependent on the low-affinity receptor for IgE, CD23, whereas the IgG2a effect depends on activating FcgammaRs. In vitro studies suggest that IgE/Ag is presented more efficiently than Ag alone to CD4(+) T cells by CD23(+) B cells and that IgG2a/Ag is presented by FcgammaR(+) dendritic cells (DCs). In this study, we investigate in vivo the early events leading to IgE- and IgG2a-mediated enhancement of immune responses. OVA administered i.v. in PBS in combination with specific IgE binds circulating B cells after 5 min and is found in B cell follicles bound to follicular B cells (CD23(high)) after 30 min. This novel B cell-dependent route of entry is specific for IgE because IgG2a-Ag complexes were trapped in the marginal zone. OVA-specific CD4(+) T cells were found at the T-B border in the T cell zones 12 h after immunization both with IgE/OVA or IgG2a/OVA and proliferated vigorously after 3 days. The findings suggest that IgE- and IgG2a-immune complexes are efficient stimulators of early CD4(+) T cell responses and that Ag bound to IgE has a specific route for transportation into follicles.
- Subjects :
- Adoptive Transfer
Animals
CD4-Positive T-Lymphocytes immunology
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Fluorescent Antibody Technique
Immunoglobulin G immunology
Immunohistochemistry
Lymphocyte Activation immunology
Male
Mice
Mice, Transgenic
Microscopy, Confocal
Ovalbumin immunology
Receptors, IgE immunology
Receptors, IgE metabolism
Trinitrobenzenes immunology
Antigen Presentation immunology
Antigen-Antibody Complex immunology
B-Lymphocytes immunology
Germinal Center immunology
Immunoglobulin E immunology
Spleen immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 180
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 18453579
- Full Text :
- https://doi.org/10.4049/jimmunol.180.10.6604