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Evaluation of viability assays for anthocyanins in cultured cells.

Authors :
Elisia I
Popovich DG
Hu C
Kitts DD
Source :
Phytochemical analysis : PCA [Phytochem Anal] 2008 Nov-Dec; Vol. 19 (6), pp. 479-86.
Publication Year :
2008

Abstract

The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay is a widely accepted cytotoxicity assay which can produce inaccurate results due to possible interference with the antioxidant property of anthocyanins. Alternative methods to the MTT assay, such as BrdU (DNA-based) and CellTiter-Glo (ATP-based) assays were evaluated to assess anthocyanin cytotoxicity, derived from blackberry in LNCaP, MCF-7 and MDA-MB-453 cell lines. The standard cell counting method was the reference assay. Greater correlation of cell viability values following anthocyanin exposure was obtained from multiple cell lines with the alternative assays when compared with cell counting. MTT and cell counting results were not always correlated, albeit this was a function of cell type. In particular, poor correlations between cell counting and MTT procedures used to assess cytotoxicity of anthocyanins were observed in the MDA-MB-453 cell lines. Comparison of cytotoxicity derived from alternative assays and the MTT assays with the cell counting method was dependent on the assay procedure and the cell type. The LC(50) of blackberry crude extract ranged from 0.4 to 9.4 mg/mL between assays and across all cell lines, whereas a semi-purified anthocyanin extract was not cytotoxic. Cytotoxicity evaluation of polyphenolic-rich extracts using BrdU and CellTiter-Glo assays as alternatives to the MTT method is recommended.

Details

Language :
English
ISSN :
1099-1565
Volume :
19
Issue :
6
Database :
MEDLINE
Journal :
Phytochemical analysis : PCA
Publication Type :
Academic Journal
Accession number :
18435529
Full Text :
https://doi.org/10.1002/pca.1069