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Synthetic strategies and parameters involved in the synthesis of oligodeoxyribonucleotides according to the phosphoramidite method.
- Source :
-
Current protocols in nucleic acid chemistry [Curr Protoc Nucleic Acid Chem] 2001 May; Vol. Chapter 3, pp. Unit 3.3. - Publication Year :
- 2001
-
Abstract
- The phosphoramidite approach has had a major impact on the synthesis of oligonucleotides. This unit describes parameters that affect the performance of this method for preparing oligodeoxyribonucleotides, as well as a number of compatible strategies. Milestones that led to the discovery of the approach are chronologically reported. Alternate strategies are also described to underscore the versatility by which these synthons can be obtained. Mechanisms of deoxyribonucleoside phosphoramidite activation, factors affecting condensation, and deprotection strategies are discussed.
Details
- Language :
- English
- ISSN :
- 1934-9289
- Volume :
- Chapter 3
- Database :
- MEDLINE
- Journal :
- Current protocols in nucleic acid chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 18428844
- Full Text :
- https://doi.org/10.1002/0471142700.nc0303s00