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Production of polyclonal antisera.

Authors :
Cooper HM
Paterson Y
Source :
Current protocols in neuroscience [Curr Protoc Neurosci] 2001 May; Vol. Chapter 5, pp. Unit 5.5.
Publication Year :
2001

Abstract

Antibodies are serum immunoglobulins with binding specificity for particular antigens, and polyclonal antibodies are particularly valuable for immunoprecipitation and immunoblotting. In this unit, the production of polyclonal antisera specific for protein antigens in rabbits, rats, mice, and hamsters is described. A support protocol is included for preparing serum from blood.

Details

Language :
English
ISSN :
1934-8576
Volume :
Chapter 5
Database :
MEDLINE
Journal :
Current protocols in neuroscience
Publication Type :
Academic Journal
Accession number :
18428504
Full Text :
https://doi.org/10.1002/0471142301.ns0505s00