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Simultaneous determination of phenylenediamine isomers and dihydroxybenzene isomers in hair dyes by capillary zone electrophoresis coupled with amperometric detection.
- Source :
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Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2008 May; Vol. 391 (2), pp. 653-9. Date of Electronic Publication: 2008 Apr 18. - Publication Year :
- 2008
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Abstract
- The simultaneous determination of three isomers of phenylenediamines (o, m, and p-phenylenediamine) and two isomers of dihydroxybenzenes (catechol and resorcinol) in hair dyes was performed by capillary zone electrophoresis coupled with amperometric detection (CZE-AD). The effects of working electrode potential, pH and concentration of running buffer, separation voltage, and injection time on CZE-AD were investigated. Under the optimum conditions the five analytes could be perfectly separated in 0.30 mol L(-1) borate-0.40 mol L(-1) phosphate buffer (pH 5.8) within 15 min. A 300 μm diameter platinum electrode had good responses at +0.85 V (versus SCE) for the five analytes. Their linear ranges were from 1.0 × 10(-6) to 1.0 × 10(-4) mol L(-1) and the detection limits were as low as 10(-7) mol L(-1) (S/N = 3). This working electrode was successfully used to analyze eight kinds of hair dye sample with recoveries in the range 91.0-108.0% and RSDs less than 5.0%. These results demonstrated that capillary zone electrophoresis coupled with electrochemical detection using a platinum working electrode as detector was convenient, highly sensitive, highly repeatable and could be used in the rapid determination of practical samples.
Details
- Language :
- English
- ISSN :
- 1618-2650
- Volume :
- 391
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical and bioanalytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 18421447
- Full Text :
- https://doi.org/10.1007/s00216-008-2053-5