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Differential expression of iutA and ibeA in the early stages of infection by extra-intestinal pathogenic E. coli.
- Source :
-
Microbes and infection [Microbes Infect] 2008 Apr; Vol. 10 (4), pp. 432-8. Date of Electronic Publication: 2008 Jan 12. - Publication Year :
- 2008
-
Abstract
- Extraintestinal pathogenic Escherichia coli strains are responsible for a number of infections in humans and animals. Several ExPEC virulence genes have already been described such as iutA involved in iron acquisition and ibeA required for invasion of eukaryotic cells. In this study we used the chicken model to study the expression of iutA and ibeA by two ExPEC strains during growth of bacteria in LB medium and during the infection. Expression of iutA and ibeA were shown to be higher in stationary phase than in exponential phase in vitro. During infection, iutA expression was increased at least 50-fold in the airsac and in the lung 3, 6 and 24h. p.i. compared to in vitro grown bacteria. Expression of ibeA was increased 2.5-9-fold in the airsac in the early stages of the infection only. This is the first report analyzing quantitatively the expression of ExPEC virulence genes during the course of the infection. The model described could be useful to study the expression of other ExPEC virulence genes.
- Subjects :
- Air Sacs microbiology
Animals
Bacterial Outer Membrane Proteins biosynthesis
Chickens
Escherichia coli Infections microbiology
Escherichia coli Proteins biosynthesis
Lung microbiology
Membrane Proteins biosynthesis
Up-Regulation
Bacterial Outer Membrane Proteins genetics
Escherichia coli genetics
Escherichia coli Proteins genetics
Gene Expression Profiling
Membrane Proteins genetics
Virulence Factors biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1286-4579
- Volume :
- 10
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Microbes and infection
- Publication Type :
- Academic Journal
- Accession number :
- 18403237
- Full Text :
- https://doi.org/10.1016/j.micinf.2008.01.002