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A novel mRNA 3' untranslated region translational control sequence regulates Xenopus Wee1 mRNA translation.
- Source :
-
Developmental biology [Dev Biol] 2008 May 15; Vol. 317 (2), pp. 454-66. Date of Electronic Publication: 2008 Feb 29. - Publication Year :
- 2008
-
Abstract
- Cell cycle progression during oocyte maturation requires the strict temporal regulation of maternal mRNA translation. The intrinsic basis of this temporal control has not been fully elucidated but appears to involve distinct mRNA 3' UTR regulatory elements. In this study, we identify a novel translational control sequence (TCS) that exerts repression of target mRNAs in immature oocytes of the frog, Xenopus laevis, and can direct early cytoplasmic polyadenylation and translational activation during oocyte maturation. The TCS is functionally distinct from the previously characterized Musashi/polyadenylation response element (PRE) and the cytoplasmic polyadenylation element (CPE). We report that TCS elements exert translational repression in both the Wee1 mRNA 3' UTR and the pericentriolar material-1 (Pcm-1) mRNA 3' UTR in immature oocytes. During oocyte maturation, TCS function directs the early translational activation of the Pcm-1 mRNA. By contrast, we demonstrate that CPE sequences flanking the TCS elements in the Wee1 3' UTR suppress the ability of the TCS to direct early translational activation. Our results indicate that a functional hierarchy exists between these distinct 3' UTR regulatory elements to control the timing of maternal mRNA translational activation during oocyte maturation.
- Subjects :
- 3' Untranslated Regions genetics
Animals
Base Sequence
Blotting, Western
DNA Primers genetics
Molecular Sequence Data
Oocytes growth & development
Oocytes metabolism
Regulatory Sequences, Ribonucleic Acid genetics
Sequence Analysis, DNA
Xenopus genetics
3' Untranslated Regions metabolism
Cell Cycle Proteins genetics
Gene Expression Regulation, Developmental genetics
Nuclear Proteins genetics
Protein-Tyrosine Kinases genetics
Xenopus physiology
Xenopus Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-564X
- Volume :
- 317
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 18395197
- Full Text :
- https://doi.org/10.1016/j.ydbio.2008.02.033