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MHC-unrestricted lysis of MUC1-expressing cells by human peripheral blood mononuclear cells.
MHC-unrestricted lysis of MUC1-expressing cells by human peripheral blood mononuclear cells.
- Source :
-
Immunological investigations [Immunol Invest] 2008; Vol. 37 (3), pp. 215-25. - Publication Year :
- 2008
-
Abstract
- Many human adenocarcinomas can be killed in vitro by targeted cytotoxic T-lymphocytes (CTL); however, major histocompatibility complex (MHC)-restrictions are typically required. The MUC1 antigen is common in many human adenocarcinomas, and is associated with a variable number of tandem repeats. It has been proposed that antigens with such repeated epitopes may be vulnerable to cytotoxic T-lymphocyte killing without MHC-restriction. Therefore, it is possible that MUC1-expressing malignant cells may be killed by targeted cytotoxic T-lymphocyte in the absence of MHC-restriction. In this study, a human MUC1-expressing murine mammary carcinoma cell line was used to determine if cytotoxic T-lymphocyte killing of MUC1-expressing adenocarcinoma cells requires MHC-restriction. Specifically, MUC1-stimulated human mononuclear cells (M1SMC) were observed to kill human MUC1-transfected, MUC1-expressing murine mammary carcinoma cells, but not the mock-transfected, non-MUC1-expressing murine mammary carcinoma cells. Furthermore, the killing was blocked by antibody to MUC1, indicating MUC1-specific killing. In conclusion, cytotoxic T-lymphocyte killing of MUC1-expressing adenocarcinoma cells can be MHC-unrestricted.
- Subjects :
- Adenocarcinoma metabolism
Animals
Antibodies, Neoplasm immunology
Antibodies, Neoplasm metabolism
Antigen-Presenting Cells immunology
Antigens, Neoplasm biosynthesis
Antigens, Neoplasm immunology
Antigens, Neoplasm metabolism
CD4-Positive T-Lymphocytes immunology
Epitopes
Female
Humans
Immunodominant Epitopes immunology
Lymphocyte Activation
Mammary Neoplasms, Animal metabolism
Mice
Mucin-1 chemistry
Transgenes
Tumor Cells, Cultured
Adenocarcinoma immunology
Cytotoxicity, Immunologic
Leukocytes, Mononuclear immunology
Major Histocompatibility Complex immunology
Mammary Neoplasms, Animal immunology
Mucin-1 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1532-4311
- Volume :
- 37
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Immunological investigations
- Publication Type :
- Academic Journal
- Accession number :
- 18389441
- Full Text :
- https://doi.org/10.1080/08820130801967874