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MHC-unrestricted lysis of MUC1-expressing cells by human peripheral blood mononuclear cells.

MHC-unrestricted lysis of MUC1-expressing cells by human peripheral blood mononuclear cells.

Authors :
Wright SE
Rewers-Felkins KA
Quinlin IS
Fogler WE
Phillips CA
Townsend M
Robinson W
Philip R
Source :
Immunological investigations [Immunol Invest] 2008; Vol. 37 (3), pp. 215-25.
Publication Year :
2008

Abstract

Many human adenocarcinomas can be killed in vitro by targeted cytotoxic T-lymphocytes (CTL); however, major histocompatibility complex (MHC)-restrictions are typically required. The MUC1 antigen is common in many human adenocarcinomas, and is associated with a variable number of tandem repeats. It has been proposed that antigens with such repeated epitopes may be vulnerable to cytotoxic T-lymphocyte killing without MHC-restriction. Therefore, it is possible that MUC1-expressing malignant cells may be killed by targeted cytotoxic T-lymphocyte in the absence of MHC-restriction. In this study, a human MUC1-expressing murine mammary carcinoma cell line was used to determine if cytotoxic T-lymphocyte killing of MUC1-expressing adenocarcinoma cells requires MHC-restriction. Specifically, MUC1-stimulated human mononuclear cells (M1SMC) were observed to kill human MUC1-transfected, MUC1-expressing murine mammary carcinoma cells, but not the mock-transfected, non-MUC1-expressing murine mammary carcinoma cells. Furthermore, the killing was blocked by antibody to MUC1, indicating MUC1-specific killing. In conclusion, cytotoxic T-lymphocyte killing of MUC1-expressing adenocarcinoma cells can be MHC-unrestricted.

Details

Language :
English
ISSN :
1532-4311
Volume :
37
Issue :
3
Database :
MEDLINE
Journal :
Immunological investigations
Publication Type :
Academic Journal
Accession number :
18389441
Full Text :
https://doi.org/10.1080/08820130801967874