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Oral tolerance attenuates changes in in vitro lung tissue mechanics and extracellular matrix remodeling induced by chronic allergic inflammation in guinea pigs.
- Source :
-
Journal of applied physiology (Bethesda, Md. : 1985) [J Appl Physiol (1985)] 2008 Jun; Vol. 104 (6), pp. 1778-85. Date of Electronic Publication: 2008 Apr 03. - Publication Year :
- 2008
-
Abstract
- Recent studies emphasize the presence of alveolar tissue inflammation in asthma. Immunotherapy has been considered a possible therapeutic strategy for asthma, and its effect on lung tissue had not been previously investigated. Measurements of lung tissue resistance and elastance were obtained before and after both ovalbumin and acetylcholine challenges. Using morphometry, we assessed eosinophil and smooth muscle cell density, as well as collagen and elastic fiber content, in lung tissue from guinea pigs with chronic pulmonary allergic inflammation. Animals received seven inhalations of ovalbumin (1-5 mg/ml; OVA group) or saline (SAL group) during 4 wk. Oral tolerance (OT) was induced by offering ad libitum ovalbumin 2% in sterile drinking water starting with the 1st inhalation (OT1 group) or after the 4th (OT2 group). The ovalbumin-exposed animals presented an increase in baseline and in postchallenge resistance and elastance related to baseline, eosinophil density, and collagen and elastic fiber content in lung tissue compared with controls. Baseline and post-ovalbumin and acetylcholine elastance and resistance, eosinophil density, and collagen and elastic fiber content were attenuated in OT1 and OT2 groups compared with the OVA group. Our results show that inducing oral tolerance attenuates lung tissue mechanics, as well as eosinophilic inflammation and extracellular matrix remodeling induced by chronic inflammation.
- Subjects :
- Administration, Inhalation
Administration, Oral
Animals
Asthma metabolism
Asthma physiopathology
Chronic Disease
Collagen metabolism
Disease Models, Animal
Elastic Tissue metabolism
Guinea Pigs
Lung metabolism
Lung pathology
Lung physiopathology
Ovalbumin administration & dosage
Pneumonia metabolism
Pneumonia physiopathology
Pulmonary Eosinophilia metabolism
Pulmonary Eosinophilia physiopathology
Pulmonary Eosinophilia prevention & control
Time Factors
Airway Resistance
Asthma immunology
Extracellular Matrix metabolism
Immune Tolerance
Lung immunology
Lung Compliance
Pneumonia immunology
Pulmonary Eosinophilia immunology
Subjects
Details
- Language :
- English
- ISSN :
- 8750-7587
- Volume :
- 104
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of applied physiology (Bethesda, Md. : 1985)
- Publication Type :
- Academic Journal
- Accession number :
- 18388250
- Full Text :
- https://doi.org/10.1152/japplphysiol.00830.2007