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Microcirculatory alterations of hepatic and mesenteric microcirculation in endotoxin tolerance.
- Source :
-
Shock (Augusta, Ga.) [Shock] 2008 Feb; Vol. 29 (2), pp. 223-31. - Publication Year :
- 2008
-
Abstract
- LPSs getting access to the circulation of mammalian organisms cause typical systemic inflammatory reactions with symptoms characteristic for acute sepsis. One possibility to attenuate LPS effects is to expose a host to a challenge with low LPS doses, which results in the establishment of "endotoxin tolerance" (ET). Because the microcirculation is of particular importance in LPS action, it seemed of interest to analyze leukocyte-endothelial interactions in the mesentery and liver once endotoxin tolerance has been established and are challenged with LPS. The mesenteric and hepatic microcirculation was investigated by intravital microscopy. After induction of ET LPS, shock was induced by i.v. injection of LPS, and microcirculation of the mesentery and liver was examined. Endotoxin tolerance resulted in reduced ex vivo TNF-alpha synthesis of whole blood. In vivo LPS caused no increase of body temperature. In sinusoids, LPS challenge increased adherence of leukocytes in naive rats, which was almost completely prevented by ET induction. In contrast, in postsinusoidal venules, leukocyte adherence was more intense after ET induction and subsequent to LPS application. Similarly, in postcapillary mesenteric venules, increased adherence of leukocytes after LPS challenge in the ET group was observed. After LPS injection, the endothelial barrier was more disturbed in the nontolerant group when compared with the ET group. Soluble L-selectin and intercellular adhesion molecule were elevated in both ET and untreated rats. Endotoxin tolerance influences leukocyte-endothelial interaction differentially depending on organ and vessel area.
- Subjects :
- Animals
Cell Adhesion drug effects
E-Selectin metabolism
Intercellular Adhesion Molecule-1 blood
L-Selectin metabolism
Leukocytes cytology
Lipopolysaccharides pharmacology
Liver blood supply
Liver metabolism
Male
Mesentery blood supply
Mesentery metabolism
Microcirculation drug effects
Rats
Tumor Necrosis Factor-alpha metabolism
Endotoxins pharmacology
Liver drug effects
Mesentery drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1073-2322
- Volume :
- 29
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Shock (Augusta, Ga.)
- Publication Type :
- Academic Journal
- Accession number :
- 18386390
- Full Text :
- https://doi.org/10.1097/shk.0b013e3180ca9ef3