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Milk fat globule membranes. Inhibition by sucrose of the alkaline phosphomonoesterase.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1976 Oct 05; Vol. 448 (2), pp. 234-44. - Publication Year :
- 1976
-
Abstract
- Sucrose, a widely used agent in the preparation of membranes, inhibited the alkaline phosphomonoesterase of the milk fat globule membrane in both its membrane-bound and detergent-solubilized forms. The inhibition was kinetically competitive and reversible by dialysis. However, its mechanism was more complex than simple competition with substrate because: (a) sucrose induced the appearance of prolonged time-lags in the progress curves of the enzyme; (b) the extent of inhibition and of the time-lags depended on the age of the membrane preparation, the period of pre-exposure of the membranes to sucrose, and the temperature of pre-exposure. On the other hand the acid phosphomonoesterase and the phosphodiesterase activities also present in the membrane preparations were unaffected by the disaccharide.
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 448
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 183835
- Full Text :
- https://doi.org/10.1016/0005-2736(76)90239-x