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Modulation of cardiac performance by motor protein gene transfer.
- Source :
-
Annals of the New York Academy of Sciences [Ann N Y Acad Sci] 2008 Mar; Vol. 1123, pp. 96-104. - Publication Year :
- 2008
-
Abstract
- Cardiac muscle performance can be determined by factors intrinsic to each cardiac muscle cell, such as protein isoform expression. One protein whose expression plays a major role in determining cardiac performance is myosin. Myosin is the heart's molecular motor which transduces the chemical energy from ATP hydrolysis into the mechanical energy of each heartbeat. Alterations of myosin isoform expression are routinely associated with acquired and inherited cases of cardiomyopathy. For example, human heart failure is consistently associated with increased expression of a slow myosin motor isoform and a concomitant decreased expression of the heart's fast myosin motor isoform. Further, mutations of the cardiac myosin gene are the most common cause of inherited hypertrophic cardiomyopathy. Transgenic animal studies have provided insight into cardiac functional effects caused by myosin isoform gene switching (fast-to-slow myosin or slow-to-fast myosin) or by expression of a disease-related mutant motor. More direct structure-function analysis using acute gene transfer of myosin motors provides evidence that the inotropic state of cardiac muscle can be affected by motor protein isoform shifting independent of intracellular calcium handling. Because most therapies for the diseased heart target intracellular calcium handling, acute gene transfer of cardiac molecular motors to modulate heart performance offers a novel therapeutic strategy for the compromised heart. Although the development of safe vectors for therapeutic myosin gene delivery are in their infancy, studies focused on acute genetic engineering of the heart's molecular motor will provide a foundation for therapeutic vector development and insight into mechanisms that contribute to cardiomyopathy.
- Subjects :
- Animals
Cardiac Myosins genetics
Cardiac Myosins physiology
Cardiomyopathies genetics
Cardiomyopathies physiopathology
Genetic Engineering methods
Humans
Protein Isoforms genetics
Protein Isoforms physiology
Ventricular Myosins genetics
Ventricular Myosins physiology
Gene Transfer Techniques
Heart physiology
Molecular Motor Proteins genetics
Molecular Motor Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0077-8923
- Volume :
- 1123
- Database :
- MEDLINE
- Journal :
- Annals of the New York Academy of Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 18375581
- Full Text :
- https://doi.org/10.1196/annals.1420.011