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Geminivirus: biolistic inoculation and molecular diagnosis.

Authors :
Santos AA
Florentino LH
Pires AB
Fontes EP
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2008; Vol. 451, pp. 563-79.
Publication Year :
2008

Abstract

The Geminiviridae family is a large family of plant viruses that has single-stranded DNA genomes and infects a large variety of crop species. In this chapter, we describe a biolistic inoculation protocol that has been successfully used to propagate new species of geminivirus in permissive hosts with total DNA extracted from infected plants. This allows us to directly investigate the biological properties of uncloned and not sap-transmissible geminiviruses.

Details

Language :
English
ISSN :
1064-3745
Volume :
451
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
18370282
Full Text :
https://doi.org/10.1007/978-1-59745-102-4_39