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Altered distribution of beta-catenin and prognostic roles in colorectal carcinogenesis.
- Source :
-
Scandinavian journal of gastroenterology [Scand J Gastroenterol] 2008; Vol. 43 (4), pp. 456-64. - Publication Year :
- 2008
-
Abstract
- Objective: Although beta-catenin cytoplasmic stabilization and nuclear translocation play a key role in initiation of colorectal cancer (CRC), the mechanisms are far from clear. The aim of this study was to investigate the relation of expressions of cyclooxygenase (COX)-2 and E-cadherin, and the beta-catenin gene exon 3 mutation to the altered distribution of beta-catenin, and their roles in CRC progression and prognosis.<br />Material and Methods: Expressions of beta-catenin, COX-2 and E-cadherin in 96 tissue specimens were detected by immunohistochemistry, and mutation of beta-catenin gene exon 3 was screened by polymerase chain reaction (PCR) and denaturing high-performance liquid chromatography (DHPLC).<br />Results: Cytoplasmic/nuclear expression of beta-catenin and reduced membranous expression of E-cadherin were associated, respectively, with the earlier and later stages of sequential colorectal carcinogenesis (p<0.05). The altered distribution of beta-catenin was significantly associated with both high Dukes' stages and poor differentiation of CRC (p<0.05). It also had a parallel relationship with COX-2 overexpression (p<0.05, Spearman's rank analysis), but not with reduced E-cadherin expression. Kaplan-Meier analysis showed a significantly worse survival rate for CRC patients with altered expression of beta-catenin (p<0.05, log-rank test). Nevertheless, we failed to find any exon 3 mutation of beta-catenin gene in all 60 cases of CRC.<br />Conclusions: Altered distribution of beta-catenin occurs in the early stage of colorectal carcinogenesis and has a parallel relationship with COX-2 overexpression. It may serve as a potential marker for the progression and prognosis of CRC. The exon 3 mutation did not appear contributive to the abnormal expression of beta-catenin in CRCs in a Chinese population.
- Subjects :
- Adult
Aged
Aged, 80 and over
Biomarkers, Tumor analysis
Cell Membrane metabolism
Cell Nucleus metabolism
Colorectal Neoplasms genetics
Colorectal Neoplasms mortality
Cyclooxygenase 2 metabolism
Cytoplasm metabolism
Female
Humans
Immunohistochemistry
Male
Middle Aged
Mutation
Prognosis
Survival Rate
beta Catenin genetics
Cadherins metabolism
Colorectal Neoplasms metabolism
beta Catenin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1502-7708
- Volume :
- 43
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Scandinavian journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 18365911
- Full Text :
- https://doi.org/10.1080/00365520701785194