Back to Search
Start Over
[Intestinal intraepithelial lymphocytes in cirrhosis: experiment with rats].
- Source :
-
Zhonghua yi xue za zhi [Zhonghua Yi Xue Za Zhi] 2008 Jan 22; Vol. 88 (4), pp. 233-5. - Publication Year :
- 2008
-
Abstract
- Objective: To investigate the phenotypes of T lymphocytes in the intestinal epithelium in cirrhosis.<br />Methods: Forty Wistar rats were randomly divided into 2 groups: cirrhosis group (n = 25) undergoing subcutaneous injection of 40% carbon tetrachloride twice a week for 10-12 weeks to establish cirrhosis models, and control group (n = 15). Twelve weeks later the rats were killed with their levers taken out. The T lymphocytes in the intestinal epithelium were isolated and labeled with mouse anti-rat CD3, CD4 and CD8 monoclonal antibody, Flow cytometry was performed.<br />Results: The number of intestinal intraepithelial lymphocytes of the cirrhosis group was (3.4 +/- 1.1) x 10(5)/cm, significantly higher than that of the control group [(2.3 +/- 0.5) x 10(5)/cm, P < 0.05]. The proportion of CD3(+) cells of the cirrhosis group were (76 +/- 8)%, not significantly different from that of the control group [(80 +/- 6)%, P > 0.05]. There were no significant different between two group (P > 0.05). The proportion of CD4(+)CD8(+) subpopulation of the cirrhosis group was (6.9 +/- 3.3)%, significantly higher than that of the control group [(3.7 +/- 1.8)%, P < 0.05]. The proportion of CD4(+)CD8(-) subpopulation of the cirrhosis group was (6.9 +/- 3.0)%, significantly higher than that of the control group [(4.4 +/- 1.4)%, P < 0.05].<br />Conclusion: There were alterations of the immune barrier function in cirrhosis. The increase of the proportion of CD4(+)CD8(+) subpopulation may be associated with the increases of the number of intestinal bacteria.
- Subjects :
- Animals
CD3 Complex immunology
CD4 Antigens immunology
CD4-Positive T-Lymphocytes immunology
CD4-Positive T-Lymphocytes pathology
CD8-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes pathology
Intestinal Mucosa immunology
Liver Cirrhosis, Experimental immunology
Lymphocyte Count
Male
Rats
Rats, Wistar
T-Lymphocytes immunology
Intestinal Mucosa pathology
Liver Cirrhosis, Experimental pathology
T-Lymphocytes pathology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0376-2491
- Volume :
- 88
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Zhonghua yi xue za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 18361832