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Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2008 May; Vol. 74 (10), pp. 3302-5. Date of Electronic Publication: 2008 Mar 21. - Publication Year :
- 2008
-
Abstract
- We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic libraries.
Details
- Language :
- English
- ISSN :
- 1098-5336
- Volume :
- 74
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 18359830
- Full Text :
- https://doi.org/10.1128/AEM.02630-07