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Selective apoptosis of breast cancer cells by siRNA targeting of BORIS.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2008 May 23; Vol. 370 (1), pp. 109-12. Date of Electronic Publication: 2008 Mar 18. - Publication Year :
- 2008
-
Abstract
- Brother of the regulator of imprinted sites (BORIS) is an epigenetically acting transcription factor which represses the tumor inhibitor functions of the tumor suppressor protein CTCF. BORIS expression has not been documented in adult females, making it an exciting molecular target for drug development in breast cancer. Previously, we demonstrated that vaccination of mice with zing-finger (ZF)-deleted non-functional BORIS results in regression of breast cancer and generation of potent anti-tumor immune responses. RNAi induction can be used as an alternative approach for selective tumor cell killing. Short interfering RNA (siRNA) molecules targeting BORIS were generated and their efficacy was tested in MDA-MB-231 breast cancer and non-malignant epithelial cell lines. Treatment with BORIS-specific siRNA, but not control siRNA led to a concentration-dependent reduction in BORIS expression and proportional apoptotic death of the cancer but not control cells. To our knowledge this is first report demonstrating a critical role of BORIS in maintaining tumor cell viability.
- Subjects :
- Base Sequence
Caspase 3 metabolism
Caspase 7 metabolism
Cell Line, Tumor
Cell Survival drug effects
Cell Survival genetics
DNA-Binding Proteins genetics
Female
Humans
Molecular Sequence Data
RNA, Small Interfering genetics
Transfection
Apoptosis genetics
Breast Neoplasms metabolism
DNA-Binding Proteins antagonists & inhibitors
RNA, Small Interfering pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 370
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 18355444
- Full Text :
- https://doi.org/10.1016/j.bbrc.2008.03.040