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Lysophosphatidylcholine induces mast cell secretion and protein kinase C activation.
- Source :
-
The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 1991 Nov; Vol. 88 (5), pp. 721-30. - Publication Year :
- 1991
-
Abstract
- Lysophosphatidylcholine (lyso-PC), a natural product of phospholipase A2 activity, induced the secretion of both granule-associated beta-hexosaminidase and newly generated leukotriene C4 from mouse bone marrow-derived mast cells. Micromolar concentrations of lyso-PC potentiated the release of beta-hexosaminidase induced by specific antigen but not the calcium ionophore, A23187. Exogenous adenosine was relatively ineffective in enhancing beta-hexosaminidase release from cells challenged with lyso PC. Lyso-PC caused a marked increase in intracellular free-calcium levels and induced the activation of protein kinase C (PKC). These effects could not be abrogated by a prolonged preincubation with pertussis toxin. Staurosporine, an inhibitor of PKC, partially inhibited the abilities of antigen and A23187 to induce beta-hexosaminidase release but was ineffective when lyso-PC was the secretagogue. Lyso-PC appears to activate mast cell PKC, but its ability to stimulate mast cell mediator release appears to be related to its ability to elevate intracellular free calcium concentrations.
- Subjects :
- Adenosine pharmacology
Alkaloids pharmacology
Animals
Bone Marrow Cells
Calcimycin pharmacology
Calcium metabolism
Cells, Cultured
Mast Cells drug effects
Mast Cells enzymology
Mice
Mice, Inbred BALB C
Protein Kinase C antagonists & inhibitors
Protein Kinase C metabolism
SRS-A metabolism
Staurosporine
beta-N-Acetylhexosaminidases metabolism
Lysophosphatidylcholines pharmacology
Mast Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0091-6749
- Volume :
- 88
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of allergy and clinical immunology
- Publication Type :
- Academic Journal
- Accession number :
- 1835466
- Full Text :
- https://doi.org/10.1016/0091-6749(91)90178-q