Back to Search
Start Over
Viruses induce high expression of BAFF by salivary gland epithelial cells through TLR- and type-I IFN-dependent and -independent pathways.
- Source :
-
European journal of immunology [Eur J Immunol] 2008 Apr; Vol. 38 (4), pp. 1058-64. - Publication Year :
- 2008
-
Abstract
- B cell activating factor (BAFF) plays a key role in promoting B lymphocyte activation. We investigated whether danger signals induce BAFF secretion by cultured salivary gland epithelial cells (SGEC), which are the target of primary Sjögren's syndrome, a prototypic systemic autoimmune disease. SGEC cultures were established from minor salivary glands obtained from ten patients with pSS or sicca symptoms. BAFF mRNA and protein were measured after stimulation of the different Toll-like receptors (TLR) by agonists or viruses. The expression of TLR2, -3, and -7 was detected in SGEC. Poly (I:C) (a synthetic TLR3 agonist) and reovirus-1 (a dsRNA virus) induced high expression of BAFF mRNA (multiplied by a factor of 246 +/- 39 (SEM) and 347 +/- 66, respectively) and of BAFF protein secretion (58.49 +/- 4.34 pg/mL and 69.73 +/- 5.67). Inhibition of both the endosomal (by chloroquine) and IFN (by anti-IFNAR antibody) pathways partly inhibited BAFF expression. Treatment with both dsRNA virus and poly (I:C) induced high levels of BAFF mRNA and protein expression by SGEC, through pathways dependent on and independent of TLR and dependent on and independent of IFN. BAFF induction by target organs of autoimmune diseases after viral infection may be a link between innate immunity and autoimmunity.
- Subjects :
- Animals
Autoimmunity immunology
B-Cell Activating Factor genetics
Cells, Cultured
Chlorocebus aethiops
Epithelial Cells metabolism
Gene Expression Regulation
Herpesvirus 1, Human immunology
Humans
Immunity, Innate immunology
Ligands
Orthoreovirus, Mammalian immunology
Salivary Glands metabolism
Vero Cells
Virus Diseases genetics
Virus Diseases immunology
Virus Diseases metabolism
B-Cell Activating Factor metabolism
Epithelial Cells immunology
Interferon Type I metabolism
Salivary Glands immunology
Signal Transduction immunology
Toll-Like Receptors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2980
- Volume :
- 38
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 18350548
- Full Text :
- https://doi.org/10.1002/eji.200738013