Back to Search
Start Over
Co-treatment of suberoylanilide hydroxamic acid and mitomycin-C induces the apoptosis of rabbit tenon's capsule fibroblast and improves the outcome of glaucoma filtration surgery.
- Source :
-
Current eye research [Curr Eye Res] 2008 Mar; Vol. 33 (3), pp. 237-45. - Publication Year :
- 2008
-
Abstract
- Purpose: This study was undertaken to develop a new treatment modality that would be able to minimize fibrosis and provide better outcome with glaucoma filtration surgery (GFS).<br />Methods: We examined whether co-treatment with mitomycin-C (MMC) and histone deacetylase inhibitor (HDACi) suberoylanilide hydroxamic acid (SAHA) efficiently induces apoptosis on rabbit Tenon's capsule fibroblasts (TCF) in vitro. We further examined the effect of co-treatment with SAHA and MMC on the alteration of IOP and the bleb survival in rabbits following GFS.<br />Results: Co-treatment of MMC and SAHA efficiently induces apoptosis in TCFs via the up-regulation of p53 and increased phosphorylation of p53 on serine 15 and 392. Also, co-treatment of SAHA and low-dose MMC decreases IOP, prolongs bleb survival, and induces apoptosis of cells under the bleb area following GFS.<br />Conclusion: This study shows that a co-treatment of SAHA and MMC could improve the outcome of GFS.
- Subjects :
- Alkylating Agents pharmacology
Animals
Blotting, Western
Caspase 3 metabolism
Cell Culture Techniques
Connective Tissue Cells drug effects
Drug Therapy, Combination
Enzyme Inhibitors pharmacology
Fibroblasts metabolism
Fibrosis prevention & control
Filtering Surgery
Fluorescent Antibody Technique, Indirect
Histone Deacetylase Inhibitors
Hydroxamic Acids pharmacology
In Situ Nick-End Labeling
Intraocular Pressure drug effects
Mitomycin pharmacology
Phosphorylation
Rabbits
Surgical Flaps
Tumor Suppressor Protein p53 metabolism
Up-Regulation
Vorinostat
bcl-Associated Death Protein metabolism
Alkylating Agents therapeutic use
Apoptosis drug effects
Enzyme Inhibitors therapeutic use
Fibroblasts pathology
Glaucoma surgery
Hydroxamic Acids therapeutic use
Mitomycin therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 0271-3683
- Volume :
- 33
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Current eye research
- Publication Type :
- Academic Journal
- Accession number :
- 18350434
- Full Text :
- https://doi.org/10.1080/02713680701875281