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Changes in calcium-dependent membrane permeability properties in mitochondria of livers from arthritic rats.
- Source :
-
Cell biochemistry and function [Cell Biochem Funct] 2008 Jun; Vol. 26 (4), pp. 443-50. - Publication Year :
- 2008
-
Abstract
- The involvement of the mitochondrial permeability transition pore (PTP) in the responses of mitochondria from adjuvant-induced arthritic rats to Ca(2+) addition was investigated. The respiratory activity, the Ca(2+)-induced osmotic swelling and the electrophoretic (45)Ca(2+) uptake were evaluated in the absence and in the presence of cyclosporin A (CsA), a well-known inhibitor of the mitochondrial PTP. The Ca(2+)-induced mitochondrial permeability transition (MPT) process occurred in mitochondria from arthritic rats even in the presence of a low Ca(2+) concentration. Whereas in the normal condition, the Ca(2+)-induced uncoupling of oxidative phosphorylation and osmotic swelling was observed in the presence of 10 or 20 microM Ca(2+) concentration, in the arthritic condition, these events occurred at 1.0 microM concentration. In addition, mitochondria from arthritic rats presented an impaired ability to accumulate (45)Ca(2+). All these effects were completely prevented by the administration of CsA. The results of the present study suggest that the higher sensitivity of mitochondria from arthritic rats to Ca(2+)-induced MPT may be an important factor in the pathogenesis of the arthritis disease.<br /> ((c) 2008 John Wiley & Sons, Ltd.)
- Subjects :
- Animals
Arthritis, Experimental metabolism
Cyclosporine pharmacology
Intracellular Membranes metabolism
Male
Mitochondria, Liver metabolism
Mitochondrial Swelling
Oxygen metabolism
Rats
Rats, Sprague-Dawley
Calcium pharmacology
Cell Membrane Permeability drug effects
Intracellular Membranes drug effects
Mitochondria, Liver drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0844
- Volume :
- 26
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cell biochemistry and function
- Publication Type :
- Academic Journal
- Accession number :
- 18348178
- Full Text :
- https://doi.org/10.1002/cbf.1461