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R5 and X4 HIV viruses differentially modulate host gene expression in resting CD4+ T cells.
- Source :
-
AIDS research and human retroviruses [AIDS Res Hum Retroviruses] 2008 Mar; Vol. 24 (3), pp. 485-93. - Publication Year :
- 2008
-
Abstract
- During HIV-1 infection, distinct biological phenotypes are observed between R5 and X4 HIV-1 strains with respect to pathogenicity and tropism. In this study, temporal changes of the expression levels of the complete human transcriptome, representing 47,000 well-characterized human transcripts, were monitored in the first 24 h during HIV-1 R5 and X4 exposition in resting primary CD4(+) T cells. We provide evidence that R5 viruses modulate, to a greater extent than X4 viruses, the level of mRNA of the resting CD4(+) T cells. Indeed, modulation of the TCR signaling and the actin organization involving the WAVE/ABI complex and the ARP2/3 complex appeared to be associated with R5 exposition. The data suggest that the ability of R5 viruses to modulate TCR-mediated actin polymerization and signaling creates a favorable environment for CD4(+) T cell activation after TCR stimulation and may partly explain why R5 is the primary strain observed early in the natural infection process.
- Subjects :
- Actin-Related Protein 2-3 Complex analysis
Actins analysis
CD3 Complex analysis
CD4-Positive T-Lymphocytes chemistry
Cells, Cultured
Humans
Myosin-Light-Chain Kinase analysis
Oligonucleotide Array Sequence Analysis
Proteome analysis
Reverse Transcriptase Polymerase Chain Reaction
Wiskott-Aldrich Syndrome Protein Family analysis
CD4-Positive T-Lymphocytes immunology
CD4-Positive T-Lymphocytes virology
Gene Expression Profiling
HIV-1 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0889-2229
- Volume :
- 24
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- AIDS research and human retroviruses
- Publication Type :
- Academic Journal
- Accession number :
- 18327980
- Full Text :
- https://doi.org/10.1089/aid.2007.0120