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Vascular endothelial growth factor expression in cultured periosteal-derived cells.
- Source :
-
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics [Oral Surg Oral Med Oral Pathol Oral Radiol Endod] 2008 May; Vol. 105 (5), pp. 554-60. Date of Electronic Publication: 2008 Mar 04. - Publication Year :
- 2008
-
Abstract
- The purpose of this study was to examine the expression of vascular endothelial growth factor (VEGF) during osteoblastic differentiation of cultured human periosteal-derived cells. Periosteal tissues were obtained from mandible during surgical extraction of lower impacted third molars. Periosteal-derived cells were introduced into cell culture. After passage 3, the periosteal-derived cells were further cultured for 42 days in an osteogenic-inductive culture medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. The alkaline phosphatase activity in the cultured periosteal-derived cells increased rapidly up to day 14, followed by decrease in activity. The Runx2 protein was expressed at day 7 and day 14, and its expression was not observed thereafter. Both VEGF(165) and VEGF(121) were expressed strongly at days 35 and 42 of culture, particularly during the later stages of differentiation. Alizarin red S-positive nodules were first observed on day 14 and then increased in number during the entire culture period. Osteocalcin and VEGF were first detected in the culture medium on day 14, and their levels increased thereafter in a time-dependent manner. These results suggest that VEGF secretion from cultured human periosteal-derived cells increases along with the mineralization process of the extracellular matrix.
- Subjects :
- Alkaline Phosphatase metabolism
Calcification, Physiologic
Cell Differentiation
Cells, Cultured
Core Binding Factor Alpha 1 Subunit biosynthesis
Humans
Osteocalcin biosynthesis
Osteogenesis
Osteoblasts metabolism
Periosteum cytology
Periosteum metabolism
Tissue Engineering methods
Vascular Endothelial Growth Factor A biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1528-395X
- Volume :
- 105
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
- Publication Type :
- Academic Journal
- Accession number :
- 18296084
- Full Text :
- https://doi.org/10.1016/j.tripleo.2007.08.018